China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (2): 93-95.

• 生物技术 • Previous Articles     Next Articles

Cloning and Sequence Analysis of the E2Gene of Bovine Viral Diarrhea Virus Strain Yak

HU Bing- feng1, LIU Ya- gang1, WANG Wen- bo1, YANG Xiao- nong1, 
YU Ji- feng2, FENG Ying- yang1, WANG Pan- pan1   

  1. (1.College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;2.Sichuan Animal Science Academy, Chengdu 610066, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

Abstract: Abstract: According to the E2 genes of bovine viral diarrhea virus (BVDV) which were obtained from GenBank, the target fragment of about 640 bp of isolate Yak was amplified by reverse transcription polymerase chain reaction (RT- PCR), cloned into pMD18- T vector and sequenced. The positive recombinant clone was identified by restriction enzyme digestion. Sequence comparison and phylogenetic analyses showed that isolate Yak fall into the BVDV genotype 1 and were distant from other subgroups. There is no insertion or variation of E2gene. It had the highest sequence identity (68.8％) with the Australia Braidwood strain. The low sequence identity with other BVDV- 1 strains indicate that strain Yak had an independent evolutional pathway.

Key words: Key words: Yak; bovine viral diarrhea virus; E2 gene; clone; sequence analysis