抗鸡传染性支气管炎病毒多克隆抗体的制备及其生物活性的鉴定

›› 2010, Vol. 37 ›› Issue (1): 65-68.

• 生物技术 • Previous Articles Next Articles

Preparation and Initial Identification of Anti-IBV Whole Virion Polyclonal Antibody

WANG Heng^1,2, LIU Bo-qi¹, YIN Hang¹, TANG Li¹, REN Xiao-feng¹, LI Guang-xing¹

(1.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China；2.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
Contact: LI Guang-xing

Abstract

Abstract: The biological activities of an anti-IBV whole virion antibody, which was obtained from the serum of rabbit by injected the commercial vaccinal strain H120 IBV, was detected by immunohistochemistry (IHC), immunofluorence technic and Western blotting assay, and the antibody titer was analysised by agar diffusion reaction and ELISA. The results revealed that this polyclonal antibody had a high antibody titer and could specifically bind with different IBV related antigens such as pET30a-IBV S1 recombinant protein, expressing product of pcDNA3.1-IBV S1 and Beaudette strain IBV.

Key words: infectious bronchitis virus (IBV); polyclonal antibody; activities detection

CLC Number:

S859.79+7

WANG Heng;LIU Bo-qi;YIN Hang;TANG Li;REN Xiao-feng;LI Guang-xing. Preparation and Initial Identification of Anti-IBV Whole Virion Polyclonal Antibody[J]. , 2010, 37(1): 65-68.

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Preparation and Initial Identification of Anti-IBV Whole Virion Polyclonal Antibody

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