抗鸡传染性支气管炎病毒多克隆抗体的制备及其生物活性的鉴定

China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (1): 65-68.

• 生物技术 • Previous Articles Next Articles

Preparation and Initial Identification of Anti-IBV Whole Virion Polyclonal Antibody

WANG Heng^1,2, LIU Bo-qi¹, YIN Hang¹, TANG Li¹, REN Xiao-feng¹, LI Guang-xing¹

(1.College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China；2.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
Contact: LI Guang-xing

Abstract

Abstract: The biological activities of an anti-IBV whole virion antibody, which was obtained from the serum of rabbit by injected the commercial vaccinal strain H120 IBV, was detected by immunohistochemistry (IHC), immunofluorence technic and Western blotting assay, and the antibody titer was analysised by agar diffusion reaction and ELISA. The results revealed that this polyclonal antibody had a high antibody titer and could specifically bind with different IBV related antigens such as pET30a-IBV S1 recombinant protein, expressing product of pcDNA3.1-IBV S1 and Beaudette strain IBV.

Key words: infectious bronchitis virus (IBV); polyclonal antibody; activities detection

CLC Number:

S859.79+7

WANG Heng;LIU Bo-qi;YIN Hang;TANG Li;REN Xiao-feng;LI Guang-xing. Preparation and Initial Identification of Anti-IBV Whole Virion Polyclonal Antibody[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(1): 65-68.

[1]	LIU Min, CHENG Huai, ZHENG Zhenzhen, ZHANG Mengsi, ZHANG Hewei, REN Jingqiang. Eukaryotic Expression and Polyclonal Antibody Preparation of N Protein of Canine Parainfluenza Virus [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(5): 2287-2294.
[2]	SI Wei, TAN Xiyu, XU Lingyun, LUO Tingrong, LI Xiaoning, GU Jinyan. Prokaryotic Expression of HA1 Protein of Swine Influenza Virus Subtype H3N2 and Preparation of Polyclonal Antibodies [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1739-1749.
[3]	HONG Jiajun, XIAO Jinhua, ZHANG Zheng, YANG Yuting, FANG Chun, HAN Xu. Effect of sod Gene Deletion on the Anti-oxidative Stress in Listeria monocytogenes 10403S [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(4): 1776-1783.
[4]	GUO Chenyun, SONG Jinxing, WANG Mengxiang, SUN Junru, YAN Ruoqian, XIE Caihua, WU Yanan, ZHANG Gaiping. Prokaryotic Expression,Purification and Polyclonal Antibodies Preparation of African Swine Fever Virus B602L Protein [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 3980-3989.
[5]	CUI Yanyan, WANG Guan, HAO Junfang, LI Zhiqiang, YU Fuchang, SHI Ke, XI Li, QI Meng. Prokaryotic Expression of Anaplasma phagocytophilum MSP4 Protein and Preparation of Its Polyclonal Antibodies [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 4036-4042.
[6]	XIANG Yong, LI Linlin, ZHANG Junqin, DONG Jiawen, HUANG Yunzhen, ZHAI Qi, XU Zhihong, LIAO Ming, SUN Minhua. Prokaryotic Expression of VP27 Protein of Goose Astrovirus Genotype 1 and Preparation and Identification of Its Polyclonal Antibody [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(6): 2576-2584.
[7]	HUANG Xinmiao, YAO Min, HE Hao, XIAO Huiping, CHEN Jingzhuo, FENG Hao, ZHANG Li, ZHENG Sunli, ZHANG Mengdie, HUANG Tinghua. Preparation and Application of Polyclonal Antibodies Against the Virulence Protein SptP in Salmonella Typhimurium [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(6): 2597-2604.
[8]	CAI Chang, CHEN Hewei, SONG Ruipeng, QIN Shaomin, LIU Jinfeng, QIN Shuying, SUN Qian, CHEN Tongjinyue, OU Changbo, LIU Xingyou, MA Ling, WU Jianmin. Bioinformatics Analysis and Preparation of Polyclonal Antibody of NSs Protein of Novel Akabane Virus [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 2015-2026.
[9]	ZHENG Shiling, CHEN Xueyang, LIU Jing, FANG Chun, LIANG Xiongyan, YANG Yuying. Effect of E3 Ubiquitin Ligase FBXL8 on the Replication of Avian Leukosis Virus Subgroup A [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(4): 1696-1705.
[10]	QIN Yifei, CHEN Rong, SHI Zhendan. Cloning of VDR Gene and Preparation and Identification of Its Polyclonal Antibody in Geese [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(3): 1194-1202.
[11]	LI Junxian, REN Tao, YANG Jian, GUO Yang, WEN Anlin, OU Deyuan. Screening of Traditional Chinese Medicine for Anti-avian Infectious Bronchitis Virus [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(3): 1241-1249.
[12]	REN Jianle, LIN Yiting, JI Kang, TAN Shanshan, CHEN Xinxin, JIN Yi, WANG Ying, NIU Sheng, LIANG Libin, LI Junping, ZHAO Yujun, TIAN Wenxia. Preparation of Polyclonal Antibody and Development of an Indirect ELISA Method for Senecavirus A VP2 Protein [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(2): 678-688.
[13]	WANG Yongfei, DENG Bowen, LIU Xiaoyan, HAERLEHA·Amantai, GUO Jiadong, ZHOU Zhengguo, CAI Jiang, LI Youwen. Prokaryotic Expression of EF-Tu Protein of Mycoplasma ovipneumoniae and Preparation of Polyclonal Antibody [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(2): 689-699.
[14]	ZHANG Xuliang, CHEN Li, ZHAO Zhigang, MA Chang, YOU Jinwei, DONG Min, YUN Shifeng. Prokaryotic Expression,Preparations of Polyclonal Antibodies and Immunogenicity Evaluation of NS1 Protein of Minute Virus of Mice [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(11): 4923-4931.
[15]	WU Jie, DU Jing, FAN Fan, REN Jinyang, LU Huipeng, ZHANG Li, LEI Xinnuo, CAO Shinuo, WU Zhi, ZHU Rui, ZHU Shanyuan. Prokaryotic Expression of N Protein of Porcine Epidemic Diarrhea Virus and Preparation of Polyclonal Antibodies [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(10): 4522-4530.

Preparation and Initial Identification of Anti-IBV Whole Virion Polyclonal Antibody

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments