Abstract: VP1 gene of foot-and-mouth virus (FMDV) AsiaⅠ was expressed in insect cell/Baculovirus expression system to lay a foundation for research of FMDV AsiaⅠ VP1 protein function and serology diagnostic method. The gene VP1 was amplified from the recombinant plasmid of FMDV AsiaⅠ by PCR and inserted into Baculovirus vector pFastBacHTA to get recombinant plasmid pFastBacHTA-VP1. The recombinant plasmid pFastBacHTA-VP1 was transformed into DH10Bac and the VP1 gene was integrated into Bacmid by site-specific transposition. Subsequently, the recombinant Bacmid-VP1 was transfected into Sf9 insect cells. The expressed product was identified by SDS-PAGE and Western blotting. The soluble fusion protein was purified by Ni-NTA affinity chromatography. ELISA analysis showed that the protein purified was reactive with the positive serum against FMDV AsiaⅠ. The successful expression of FMDV AsiaⅠ VP1 gene in insect cell/Baculovirus expression system provide material for research of antigenicity of AsiaⅠ VP1 protein as well as the surveillance of serology antibody.

Key words: foot-and-mouth disease virus AsiaⅠ; VP1 gene; Baculovirus; eukaryotic expression