抗CP型、NCP型牛病毒性腹泻病毒高免卵黄抗体的制备

China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (6): 124-127.

• 疾病防治 • Previous Articles Next Articles

Preparation of IgY against Cytopathic (CP) and Noncytopathic (NCP) Bovine Viral Diarrhea Virus (BVDV)

ZHAO Yu-long^1，2, WU Mo-er³, BO Xin-wen², LI Yan⁴, ZHONG Fa-gang², LI Na ^1，2, KU Chao-feng^1，2

(1.College of Animal Science and Technology, Shihezi University, Shihezi 832003, China; 2.The Breed and Biotechnology Key Laboratory of Sheep in Xinjiang Bingtuan, Shihezi 832000, China; 3.143 Agglomerate of Ziniquan Animal Husbandry 3th Company, Shihezi 832000, China; 4.Animal and Veterinary Station of Xinjiang Production and Construction Group, Urumqi 830063, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-06-20 Published:2009-06-20
Contact: ZHONG Fa-gang

Abstract

Abstract: Layer hens were immunized with cytopathic (CP) and noncytopathic (NCP) bovine viral diarrhea virus(BVDV), the egg yolk was successfully treated with modified PEG6000 method. The purity of IgY was detected with SDS-PAGE and the IgY titer was assayed by indirected ELISA after 7 days, and the neutralizing antibody titer to NCP BVDV with immunoperoxidase monolayer assay (IPMA). The results suggested that higher purity of lgY was observed with the use of modified PEG6000. We can detect anti-BVDV IgY in the first 14 days. After being immunized with 4 doses of the conjugate, the titer of IgY against CP BVDV was 1∶32000, against NCP BVDV was 1∶40000 and kept the conditions after 3 months. Neutralizing antibody titer to NCP BVDV was 1×10^-3.

Key words: BVDV; IgY; indirect ELISA; non-cell pathogenicity

CLC Number:

S852.4⁺3

ZHAO Yu-long;WU Mo-er;BO Xin-wen;LI Yan;ZHONG Fa-gang;LI Na;KU Chao-feng;. Preparation of IgY against Cytopathic (CP) and Noncytopathic (NCP) Bovine Viral Diarrhea Virus (BVDV)[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(6): 124-127.

[1]	PAN Xiangying, ZENG Zhiyong, LIANG Haiying, TANG Deyuan, WANG Bin, YE Ni, TIAN Hongli, BIAN Mengting, LIU Jiajia, HUANG Shu. Truncated Expression of Porcine Rotavirus VP6 Protein and Establishment of an Indirect ELISA Antibody Detection Method [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1231-1240.
[2]	LIN Weishan, MA Doudou, LEI Mengtong, WEI Bin, LI Guocai, WANG Guanghua, WANG Geping, JIAN Yingna, LI Xiuping. Epidemiological Investigation and Genetic Evolution Analysis of 5'-UTR of Bovine Viral Diarrhea Virus in Yaks in Haibei，Qinghai Province [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(3): 1241-1249.
[3]	LIU Zhao, DONG Qianqian, WU Aodi, WANG Kaiyue, LIANG Chengzhe, Adnan ALI, SHENG Jinliang. Isolation, Identification and Mouse Pathogenicity Analysis of a Strain of Bovine Viral Diarrhea Virus from Imported Fetal Bovine Serum [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 4052-4059.
[4]	GAO Le, SI Duoduo, GUO Lei, CHEN Can, WANG Wei, WANG Jianlin, WANG Lingling, LI Jidong. Prokaryotic Expression of GA Module-containing Protein in Mycoplasma synoviae and Establishment of Indirect ELISA Method [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(6): 2621-2632.
[5]	REN Jianle, LIN Yiting, JI Kang, TAN Shanshan, CHEN Xinxin, JIN Yi, WANG Ying, NIU Sheng, LIANG Libin, LI Junping, ZHAO Yujun, TIAN Wenxia. Preparation of Polyclonal Antibody and Development of an Indirect ELISA Method for Senecavirus A VP2 Protein [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(2): 678-688.
[6]	ZHANG Yichen, SUN Mingjun, DENG Changshun, DING Jiabo, XU Baoliang, CHEN Lei, QUAN Chunlan, ZHAO Kang, FAN Xuezheng, QIN Tong. Development and Evaluation of an Indirect ELISA Based on Recombinant E2 Protein for Detection of Bovine Viral Diarrhea Virus Antibody [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(12): 5459-5469.
[7]	XU Haojun, LIU Ying, MEI Chen, XU Tong, LI Kai, WANG Hongjun. Development of an Indirect ELISA for Antibody Detection Against Ornithobacterium rhinotracheale Based on Outer Membrane Proteins [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(10): 4420-4428.
[8]	WANG Cheng, XIA Yingju, WANG Haidong, LIU Yebing. Prokaryotic Expression of P54 Protein of African Swine Fever Virus and Establishment of an Indirect ELISA Detection Method [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(10): 4440-4449.
[9]	BIJunming, DONG Yaqin, CUIJin, SHA Zhou, GU Congcong, ZHENG Hui, WEI Rong, SUN Fuliang, ZHANG Feng, ZHANG Zhihong. Isolation，Identification and Genetic Evolution Analysis of a Strain of Bovine Viral Diarrhea Virus in Shandong Province [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(10): 4485-4499.
[10]	ZHANG Fangyuan, LIN Yating, YANG Dawei, CHEN Hu, LI Guimei, SHAN Hu. Prokaryotic Expression of African Swine Fever Virus P30 Protein and Establishment of Indirect ELISA Detection Method [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(5): 2012-2022.
[11]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(3): 1150-1159.
[12]	NIU Xiaojie, XU Mingguo, XIAO Li, LIU Wenhao, CHEN Chuangfu, WANG Yong. Expression and Purification of IPAJ Protein of Salmonella Pullorum, Preparation of Polyclonal Antibody and Establishment of ELISA Method [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(3): 1218-1228.
[13]	ZHANG Yan, ZHAN Liyuan, JIANG Fujie, MA Hongxia, KONG Lingcong. Prokaryotic Expression, Polyclonal Antibody Production and Development of an Indirect ELISA Antibody Detection Method of Recombinant Protein P48 of Mycoplasma bovis [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(11): 4621-4631.
[14]	ZHANG Kai, ZHANG Chunping, GE Shengqiang, SUN Chunxi, CHENG Jie, FU Chunyu, WANG Gang, NIU Xing, PENG Jun. Expression of p22 Protein of African Swine Fever Virus,Preparation of Polyclonal Antibody and Establishment of Indirect ELISA Method [J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(1): 270-279.
[15]	HAN Rui, YANG Xing, ZHANG Wenbo, CHEN Jialing, ZHOU Xiaoli. Prokaryotic Expression of Autolysin from Streptococcus suis and Establishment of an Indirect ELISA Method for Detection of Its Antibody [J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(9): 3508-3519.

Preparation of IgY against Cytopathic (CP) and Noncytopathic (NCP) Bovine Viral Diarrhea Virus (BVDV)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments