Abstract:

We got the PrP gene of 28 black and white cows by PCR using the primers designed according to the reported cow PrP gene. Sequence analysis showed the segment of cow PrP gene were 677 bp, coding 225 amino acids. The analysis of polymorphism of 136,154,171 amino acid site of prion protein revealed the genotype of ARQ associated with an intermediate susceptibility to TSE was present in 82.9% of cow examined, the genotype of ARR that associated with the resistance to TSE was present in 7.1% of cow examined, the genotype of VRQ associated with high susceptibility to TSE was present in 10% of cow examined. It suggested that the cow in our country was intermediate susceptibility to TSE, which could support the basic data to prevent and control the TSE in our country.

Key words: blace and white cow; genotype of PrP gene; polymorphism analysis