Abstract: In December 2008, there occurred a serious disease that nearly 100 Rhizomys sinensis died in a group of 200 Rhizomys sinensis in Guangdong province. According to the epidemiological investigation, clinical symptoms, autopsy lesion, morphology, biochemical and mice inoculution test, a Rhizomys sinensis Pasteurella strain was isolated and confirmed. It was demonstrated that the isolate strain was highly sensitive to Penicillin, Norfloxacin, Ofloxacin, Ciprofloxacin, CephalosporinⅥ in drug sensitivity test and resistant to other antibiotics，such as Kanamycin, Gentamicin, Lincomycin. Finally, the outbreak disease has been controlled timely with medication of high-sensitivity. It provided the diagnosis for the Rhizomys sinensis pasteurellosis in clinic.

Key words: Rhizomys sinensis; pasteurellosis; diagnosis and treatment