Abstract: Fowl infectious bursal disease(IBD) eleoadjuvant deactivation vaccine was prepared from bursa of sick chick. And it was used to immune health and adult rabbit by multidrop hypodermic injection to get rabbit antiIBDV serum. Hypsoexempt IgG was isolated and purified from the serum. The antibody titer was 1∶128 in rabit serums, the protein contents were 40.3 to 48.8 mg/ml; The antibody titer was 1∶32 after purification, the protein contents were 18.0 to 23.0 mg/ml. Cases of doubtful fowl IBD in several regions of Anhui province were effectively diagnosised with this agent. It provided hightiter antibody to detect IBDV antigen in AGP and immune histochemistry.

Key words: infectious bursal disease（IBD）; hyperimmune serum; salting out method; polyacrylamide gel electrophoresis(PAGE)