›› 2008, Vol. 1 ›› Issue (8): 40-43.

• 生物技术 • Previous Articles     Next Articles

Cloning and Sequencing of VP1 Gene of Feline Parvovirus CC1 Strain

LI Shuang1,2,YUAN Bao1，XIAO Shu-qi3, REN Wen-zhi1，ZHANG Jia-bao1，
ZHAO Zhi-hui2，YONG Hai-ping1,2   

  1. 1.Laboratory Animal Center of Jilin University, Changchun 130062, China; 2.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China; 3.College of Life Science,Zhongshan University, Guangzhou 510275,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-08-20 Published:2008-08-20

Abstract: To analyze the sequence of VP1 gene of Feline parvovirus CC1 strain and compare with that of representative virus strain. According to the reported complete nucleotide sequence of FPV(CU-4) in GenBank ,a pair of primers were designed and synthesized.VP1 gene of isolate was amplified.The products of PCR were cloned into PGM-T vector.the positive clone was identified bu enzyme cutting,PCR and sequencing.The whole sequence of VP1gene was analyzed using DNAStar software.The results indicated that the VP1 gene with 2306 bp long had a single opening read frame 2184 bp in length and coded a polypeptide of 727 amino acids.The sequence analysis demonstrated that represent reference strains loaded down from GenBank,the result of ORF sequence analysis indicated that CC-1 strain exhibited 99.3% homology of the nucleotides and amino acid with that of the XJ-1 strain in our county. To analyze the sequence of VP1 full gene,have a significance in clinic and epidemiological study.

Key words: feline parvovirus; VP1 gene; sequencing analysis

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