Abstract: From 150 dead poultry which have representative colibacillosis at the veterinary station animal clinic in eight counties , Huai'an. 112 colibacillosis are separated and identified. There are 92 case-hardened and 24 serogroups which includes 20 O₁₈ isolates, 14 O₇₈ isolates,11 O₁ isolates,10 O₅isolates. The four serogroups colibacillosis take the percentage 60% of 92 isolates.Antibiotic susceptibility shows that colibacillosis separated have higher drug resistance to Fluoroquinolones,Sulfamethoxazole, Trimethoprim-sulfamethpxazole, Ampicillin, Amoxilline, and also definite character to Gentamicin, Kanamycin, Cetemezin, however, it has only 3%～16.3% percentage of drug resistance to Cefalexin, Amikacin, Amoxilline-clavulanic acid, Florfenicol and Phosphonomycin.