Abstract: Realtime fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, and due to its high sensitivity, accuracy, and specificity. This technique can be classified into SYBR GreenⅠ, TaqMan, molecular beacon, complex probes and other methods based on the action modes of fluorescences. Some of the methods' principles, advantages as well as disadvantages were described in this paper respectively, and they can be applied in different fields of animal and veterinary sciences according to their different characters so as to acquire the best detection efficiency. 

Key words: real-time fluorescent quantitative PCR; fluorescence resonance energy transfer; fluorescence probes; quenching probes 