白酒糟饲粮中添加复合酶制剂对肉鸭生长、肠道发育、肉品质及养分利用的影响

doi:10.16431/j.cnki.1671-7236.2024.12.015

Abstract

Abstract: 【Objective】 This experiment was conducted to investigate the effects of compound enzyme preparation in distillers’ grains diet on growth performance,meat quality,intestinal development,blood indexes and apparent nutrient availability of meat ducks.【Method】 A total of 144 1-day-old male Peking ducks with similar body weight were randomly divided into 3 groups with 6 replicates per group and 8 ducks per replicate.Ducks in the control group were fed a corn-soybean meal basal diet,ducks in the distiller’s grains group were fed a diet supplemented with 18% distiller’s grains,and ducks in the compound enzyme preparation group were fed a diet supplemented with 500 mg/kg compound enzyme preparation on the basis of distiller’s grains group.The experimental period was divided into two feeding stages of 1-14 days and 15-35 days.After the experiment,blood,pectoral muscle,excreta and small intestine were collected,and the quality of pectoral muscle,blood indexes and apparent availability of nutrients were measured.【Result】 ①Compared with the control group, the 14-day body weight and 1-14 day ADG of ducks in distiller’s grains group and compound enzyme preparation group were significantly decreased, and the 1-14 day F/G of ducks in compound enzyme preparation group was significantly increased (P＜0.05), and the 1-14 day F/G of ducks in compound enzyme preparation group was significantly lower than that in distiller’s grains group (P＜0.05). Compared with the control group, ADFI and F/G of ducks in distilling grains group and compound enzyme preparation group were significantly increased from 15 to 35 days and from 1 to 35 days (P＜0.05), and ADFI and F/G of ducks in compound enzyme preparation group were significantly lower than those in distilling grains group from 1 to 35 days (P＜0.05). ② Compared with the control group, the chest muscle percentage of ducks in compound enzyme preparation group was significantly decreased (P＜0.05), the abdominal fat percentage of ducks in distiller’s grains group and compound enzyme preparation group was significantly increased (P＜0.05), the pancreas index of ducks in distiller’s grains group and compound enzyme preparation group, and the muscle stomach index of ducks in compound enzyme preparation group were significantly increased (P＜0.05). ③ Compared with the control group, the relative weights of duodenum and jejunum of ducks in distiller’s grains group and compound enzyme preparation group were significantly increased (P＜0.05). ④ Compared with the control group, the energy utilization rate of ducks in distiller’s grains group and compound enzyme preparation group was significantly decreased (P＜0.05). Compared with distiller’s grains group, crude protein utilization rate and energy utilization rate of ducks in compound enzyme preparation group were significantly increased (P＜0.05). ⑤ Compared with the control group, pH_{24 h} and drip loss of breast muscle of ducks in distiller’s grains group and pH_{24 h} of breast muscle of ducks in compound enzyme preparation group were significantly increased (P＜0.05), and pH_{24 h} of breast muscle of ducks in compound enzyme preparation group was significantly higher than that in distiller’s grains group, while drip loss was significantly lower than that in distiller’s grains group (P＜0.05). Compared with the control group, the pH_{45 min} of the two experimental groups was significantly decreased (P＜0.05). ⑥ There were no significant differences in blood routine indexes among groups (P>0.05). Compared with the control group, the activities of AST and LDH, AST/ALT, ALB and UA in plasma of ducks in distiller’s grains group were significantly decreased (P＜0.05), and AST/ALT in compound enzyme preparation group was significantly decreased (P＜0.05). Compared with distiller’s grains group, the contents of GLU, TP, GLB and UA in plasma of ducks in compound enzyme preparation group were significantly increased (P＜0.05).【Conclusion】 In conclusion,the compound enzyme preparation could reduce the feed/gain and improve nutrient utilization rate when the basal diet of Beijing ducks was supplemented with 18% distiller’s grains.

Key words: distiller’s grains; meet ducks; growth performance; meat quality; intestinal development; apparent digestibility of nutrients

CLC Number:

S834⁺.81

LU Zhentao, WU Zhanyue, GUO Yanhong, WANG Ning, REN Wenwen, WU Yongbao, CAO Junting, WU Xuezhuang, WEN Zhiguo. Effects of Compound Enzyme Preparation Added to Distiller’s Grains Diet on Growth, Intestinal Development,Meat Quality and Nutrients Utilization in Meat Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(12): 5265-5276.

References

[1] 浦华, 杨静, 王永伟, 等.保障国家粮食安全的蛋白替代战略构想[J].中国工程科学, 2023, 25(4):149-157.PU H, YANG J, WANG Y W, et al.Protein substitution strategy for ensuring national food security in China [J].Strategic Study of CAE, 2023, 25(4):149-157.(in Chinese)
[2] 刘长全, 韩磊, 李婷婷, 等.大食物观下中国饲料粮供给安全问题研究[J].中国农村经济, 2023，1:33-57.LIU C Q, HAN L, LI T T, et al.The security of feed grains supply in China from the perspective of a big food concept [J].Chinese Rural Economy, 2023，1:33-57.(in Chinese)
[3] 侯水生, 刘灵芝.2022年水禽产业现状、未来发展趋势与建议[J].中国畜牧杂志, 2023, 59(3):274-280.HOU S S, LIU L Z.Status quo, future development trend and suggestions of waterfowl industry in 2022[J].Chinese Journal of Animal Science, 2023, 59(3):274-280.(in Chinese)
[4] CHISORO P, JAJA I F, ASSAN N.Incorporation of local novel feed resources in livestock feed for sustainable food security and circular economy in Africa[J].Frontiers in Sustainability, 2023, 4:1251179.
[5] 耿爽, 王斯佳.非常规饲料资源开发的思路与方法[J].饲料研究, 2020, 43(5):97-100.GENG S, WANG S J.Research ideas and methods on development of unconventional feed resource [J].Feed Research, 2020, 43(5):97-100.(in Chinese)
[6] 张永刚, 张自强, 杨琦, 等.白酒糟稻壳分离及产沼气性能研究[J].中国沼气, 2023, 41(1):88-92.ZHANG Y G, ZHANG Z Q, YANG Q, et al.Study on the separation of rice husks from white distiller’s grains and the performance of biogas production[J].China Biogas, 2023, 41(1):88-92.(in Chinese)
[7] 时伟, 郑红梅, 何珺珺, 等.白酒糟中功能因子及其提取技术的研究进展[J].中国酿造, 2020, 39(7):1-6.SHI W, ZHENG H M, HE J J, et al.Research progress of functional factors and extraction techniques in distilling grains[J].China Brewing, 2020, 39(7):1-6.(in Chinese)
[8] WANG J, KONG B, FENG J, et al.A novel strategy for comprehensive utilization of distillers’ grain waste towards energy and resource recovery[J].Process Biochemistry, 2022, 113:141-149.
[9] 曾法兵, 曾益, 陈晓兰.纤维素分解菌发酵白酒糟饲喂蛋鸡试验研究[J].饲料博览, 2018，11:35-38.(in Chinese)ZENG F B, ZENG Y, CHEN X L.The effect of celulolytic-bacteria-fermented distil er grain on the layer performance[J].Feed Review, 2018，11:35-38.(in Chinese)
[10] ABD EL-HACK M E, MAHROSE K M, ATTIA F A M, et al.Laying performance, physical, and internal egg quality criteria of hens fed distillers dried grains with solubles and exogenous enzyme mixture[J].Animals, 2019, 9(4):150.
[11] ABD EL-HACK M E, CHAUDHRY M T, MAHROSE K M, et al.The efficacy of using exogenous enzymes cocktail on production, egg quality, egg nutrients and blood metabolites of laying hens fed distiller’s dried grains with solubles[J].Journal of Animal Physiology and Animal Nutrition, 2018, 102(2):e726-e735.
[12] 徐建, 陈代文, 毛倩, 等.白酒糟的营养价值评定[J].中国畜牧杂志, 2012, 48(7):47-50.XU J, CHEN D W, MAO Q, et al.Evaluation of nutritional value of distiller’s grains[J].Chinese Journal of Animal Science, 2012, 48(7):47-50.(in Chinese)
[13] 张慧, 魏来, 刘正亚, 等.发酵白酒糟营养价值的评定及其对育肥猪生长性能的影响[J].中国饲料, 2019，3:41-45.ZHANG H, WEI L, LIU Z Y, et al.The evaluation of nutritive value of fermented liquor and its effect on the growth performance of fatten pig [J].China Feed, 2019，3:41-45.(in Chinese)
[14] PEDERSEN M B, DALSGAARD S, KNUDSEN K B, et al.Compositional profile and variation of distillers dried grains with solubles from various origins with focus on non-starch polysaccharides[J].Animal Feed Science and Technology, 2014, 197:130-141.
[15] HUSAIN Q, ULLAH M F.Biocatalysis[J].Springer, 2019, 1007:973-978.
[16] MORGAN N, BHUIYAN M M, HOPCROFT R.Non-starch polysaccharide degradation in the gastrointestinal tract of broiler chickens fed commercial-type diets supplemented with either a single dose of xylanase, a double dose of xylanase, or a cocktail of non-starch polysaccharide-degrading enzymes[J].Poultry Science, 2022, 101(6):101846.
[17] 周昕.淀粉酶对大米支链淀粉酶解作用机理的研究[D].合肥：安徽农业大学, 2019.ZHOU X.Study on the mechanism of amylase hydrolysis of rice amylopectins[D].Hefei：Anhui Agricultural University, 2019.(in Chinese)
[18] 孟成明, 刘子昱, 慕春龙, 等.饲粮中添加蛋白酶对猪生长性能、营养物质消化率和血清生化指标影响的Meta分析[J].饲料工业, 2024, 45(4):30-37.MENG C M, LIU Z Y, MU C L, et al.Meta-analysis on the effect of adding protease enzymes to pig feed on growth performance，nutrient digestibility, and serum biochemical，indicators [J].Feed Industry, 2024, 45(4):30-37.(in Chinese)
[19] 田璐.白酒糟、发酵白酒糟对樱桃谷肉鸭的饲用价值研究[D].广州：华南农业大学, 2017.TIAN L.Nutrient evaluation of distiller’s grains, fermented distiller’s grains and the application in Cherry Valley ducks diets[D].Guangzhou：South China Agricultural University, 2017.(in Chinese)
[20] 庄蕾, 赵子涛, 吴永保, 等.填饲对北京鸭生长发育、胸肌生化指标和肉品质的影响[J].中国畜牧兽医, 2023, 50(8):3093-3101.ZHUANG L, ZHAO Z T, WU Y B, et al.Effects of overfeeding on growth and development, biochemical indexes and meat quality of breast muscle in Pekin ducks [J].China Animal Husbandry & Veterinary Medicine, 2023, 50(8):3093-3101.(in Chinese)
[21] 邱水玲, 杨雅妮, 靳伟刚, 等.畜禽饲用酶制剂的研究进展[J].饲料研究, 2022, 45(21):137-141.QIU S L, YANG Y N, JIN W G, et al.Research progress of forage enzyme preparations for livestock and poultry[J].Feed Research, 2022, 45(21):137-141.(in Chinese)
[22] 刘胜军, 王帅杰, 张成杰, 等.复合酶制剂在肉鸡低蛋白日粮中的应用研究[J].饲料研究, 2023, 46(11):40-46.LIU S J, WANG S J, ZHANG C J, et al.Application of compound enzyme preparation in low protein diet of broilers[J].Feed Research, 2023, 46(11):40-46.(in Chinese)
[23] PARK J, CAREY J.Dietary enzyme supplementation in duck nutrition:A review[J].Journal of Applied Poultry Research, 2019, 28(3):587-597.
[24] 张伟, 王晓亮, 张庆丽, 等.复合酶制剂对肉鸡生长性能、血清生化指标、抗氧化性能和肠道结构的影响[J].中国饲料, 2023，15:69-75.ZHANG W, WANG X L, ZHANG Q L, et al.Effects of compound enzyme preparations on growth performance, serum biochemical indices, antioxidant properties, and intestinal structure of broilers[J].China Feed, 2023，15:69-75.(in Chinese)
[25] FOUAD A M, EL-SENOUSEY H K.Nutritional factors affecting abdominal fat deposition in poultry:A review[J].Asian-Australas Journal of Animal Sciences, 2014, 27(7):1057-1068.
[26] HE R, YANG Y, LI Y, et al.Recent progress in distille’s grains:Chemical compositions and biological activities[J].Molecules, 2023, 28(22):7492.
[27] SILVA A F R, BRASIL Y L, KOCH K, et al.Resource recovery from sugarcane vinasse by anaerobic digestion—A review[J].Journal of Environmental Management, 2021, 295:113137.
[28] YAKIMOV O A, GAINULLINA M K, AKSAKOV D V, et al.Influence of poly-enzyme preparation “Universal” on the meat productivity of Cherry-Valley ducks[J].BIO Web of Conferences, 2020, 27(5)：00034.
[29] SILVA B C R, SBARDELLA M, CORASSA A, et al.Dietary high-protein distiller’s dried grains with solubles can fully replace soybean meal in diets for meat quails without affecting growth performance[J].British Poultry Science, 2023, 64(2):252-258.
[30] 吴昊.枫叶鸭与樱桃谷鸭生产性能、养分利用率及肠道消化生理的比较研究[D].雅安：四川农业大学, 2014.WU H.Comparative study on performance, nutrient utilization, and intestinal digestive physiology between Maple Leaf ducks and Cherry Valley ducks[D].Ya’an：Sichuan Agricultural University, 2014.(in Chinese)
[31] 徐浩, 江友峰, 谭洪伟, 等.酒糟中功能性成分综合利用研究进展[J].食品与发酵工业, 2023，18(2):1-11.XU H, JIANG Y F, TAN H W, et al.Research progress on comprehensive utilization of functional components in distillers grains[J].Food and Fermentation Industries, 2023, 18(2):1-11.(in Chinese)
[32] 来金良, 吕尊周, 姚玥州, 等.饲粮粗纤维水平对中新鸭生长性能、屠宰性能、肠道发育及盲肠菌群结构的影响[J].动物营养学报, 2023, 35(12):7805-7816.LAI J L, LYU Z Z, YAO Y Z, et al.Effects of dietary crude fiber levels on growth performance, slaughter performance, intestinal development and cecal flora structure of Zhongxin ducks[J].Chinese Journal of Animal Nutrition, 2023, 35(12):7805-7816.(in Chinese)
[33] LIU W.Correlation between routine blood indexes and disease activity in patients with inflammatory bowel disease[J].World Chinese Journal of Digestology, 2013, 21:3654.
[34] 范丽娟.苏淮猪血液及肠道黏膜屏障与氧化应激指标对不同纤维水平日粮的响应[D].南京：南京农业大学, 2021.FAN L J.Response of blood and intestinal mucosal barrier as well as oxidative stress related parameters in Suhuai finishing pigs to diets with varying levels of defatted rice bran[D].Nanjing：Nanjing Agricultural University, 2021.(in Chinese)
[35] 宋琼莉, 宋文静, 陈小连, 等.饲粮代谢能水平对宁都黄鸡生长性能、屠宰性能和血液生化指标的影响[J].中国畜牧兽医, 2022, 49(2):539-547.SONG Q L, SONG W J, CHEN X L, et al.Effects of dietary ME levels on growth performance, slaughter performance and blood biochemical parameters of Ningdu Yellow chickens[J].China Animal Husbandry & Veterinary Medicine, 2022, 49(2):539-547.(in Chinese)
[36] 刘杰, 郭荣, 张娟, 等.能量与蛋白质水平对静原鸡生长性能、屠宰性能、血液生化指标及代谢组的影响[J].西南农业学报, 2024, 37(3):664-677.LIU J, GUO R, ZHANG J, et al.Effects of energy and protein levels on growth performance, slaughter performance, blood biochemical parameters and metabolome of static chicken[J].Journal of Southwest Agricultural University, 2024, 37(3):664-677.(in Chinese)
[37] KHALED A Y, PARRISH C A, ADEDEJI A.Emerging nondestructive approaches for meat quality and safety evaluation—A review[J].Comprehensive Reviews in Food Science and Food Safety, 2021, 20(4):3438-3463.
[38] 孙晓明, 张佳程, 卢凌, 等.牛胴体部位肉营养成分和理化指标差异性分析[J].中国畜牧兽医, 2011, 38(2):205-208.SUN X M, ZHANG W C, LU L, et al.Analysis of differences in nutrient composition and physical and chemical indices of beef carcass parts[J].China Animal Husbandry & Veterinary Medicine, 2011, 38(2):205-208.(in Chinese)
[39] 程志斌, 廖启顺, 张红兵, 等.复合非淀粉多糖酶对肉鸡肉品质的影响[J].西南农业学报, 2012, 25(2):691-696.CHENG Z B, LIAO Q S, ZHANG H B, et al.Effect of compound non-starch polysaccharide enzymes on meat quality of broilers[J].Journal of Southwest Agricultural University, 2012, 25(2):691-696.(in Chinese)
[40] CHENG Z, ZHANG H, SU Z, et al.Effects of non-starch polysaccharide enzyme products on meat performance in broilers.ID - 20113311000[J].Animal Husbandry and Feed Science, 2011, 3(3):4-6.
[41] RAVINDRAN V, ABDOLLAHI M R.Nutrition and digestive physiology of the broiler chick:State of the art and outlook[J].Animals, 2021, 11(10):2795.
[42] STOJANOVI AĆ O, ALTIRRIBA J, RIGO D, et al.Dietary excess regulates absorption and surface of gut epithelium through intestinal PPARα[J].Nature Communications, 2021, 12(1):7031.
[43] CASPARY W F.Physiology and pathophysiology of intestinal absorption[J].The American Journal of Clinical Nutrition, 1992, 55(1 Suppl):299S-308S.
[44] 刘伟, 夏磊, 王江水, 等.饲用复合酶制剂对肉鸡生长性能、血清生化指标及肠道形态和消化酶活性的影响[J].中国畜牧杂志, 2019, 55(10):98-102.LIU W, XIA L, WANG J S, et al.Effects of feeding compound enzyme preparation on growth performance, serum biochemical indexes, intestinal morphology and digestive enzyme activities of broilers[J].Chinese Journal of Animal Science, 2019, 55(10):98-102.(in Chinese)
[45] 仵天培.果寡糖、NSP酶和益生菌对三黄鸡生产性能、血清指标、肠道形态和菌群的影响[D].南宁：广西大学, 2013.WU T P.Effects of fructo-oligosaccharides, NSP enzymes and probiotics on performance, serum indexes, intestinal morphology and microbiota of Sanhuang chickens[D].Nanning：Guangxi University, 2013.(in Chinese)

Effects of Compound Enzyme Preparation Added to Distiller’s Grains Diet on Growth, Intestinal Development,Meat Quality and Nutrients Utilization in Meat Ducks

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