基于多组学解析布鲁氏菌BvfA对睾丸支持细胞线粒体NADH-泛醌氧化还原酶复合体的影响

doi:10.16431/j.cnki.1671-7236.2024.04.008

Abstract

Abstract: 【Objective】 This study was aimed to investigate the damage effect of Brucella virulence factor A (BvfA) in Sertoli cells (TM4 cells),so as to reveal the pathogenic mechanism of Brucella on host.【Method】 RNA-Seq double-end sequencing,Label-free proteomics and LC-MS non-target metabolomics were used to analyze the omics difference of TM4 cells infected with Brucella S19△bvfA and S19 strains.The differentially expressed proteins and differential metabolite pathways were analyzed using KEGG database,and the protein expression were validated by parallel reaction monitoring (PRM).【Result】 There were 400 differentially expressed genes,422 differentially expressed proteins and 271 differential metabolites in TM4 cells infected with S19△bvfA and S19 strains.Multi-omics analysis showed that the common KEGG pathway of differentially expressed genes,proteins and metabolites in TM4 cells infected with S19△bvfA and S19 strains was retrograde endocannabinoid signaling pathway.In this pathway,20 proteins expression was down-regulated in mitochondrial NADH-ubiquinone redoxase Complex Ⅰ of TM4 cells infected with S19△bvfA strain.PRM result showed that the expression trends of Ndufb11,Ndufa9,Ndufv1,Ndufv2,Ndufs8 and Ndufs2 in Complex Ⅰ were consistent with the results of Label-free proteomics.【Conclusion】 BvfA induced variations of transcriptional,protein and metabolic levels in Brucella infected TM4 cells,and BvfA down-regulated the expression of NADH-ubiquinone oxidoreductase complex proteins in mitochondria of Sertoli cells.

Key words: Brucella; multi-omics; BvfA; Sertoli cells

CLC Number:

S852.61⁺4

SONG Qingshan, YANG Jiangliu, LIU Jun, JIA Fang. Multi-omics Analysis of the Effect of Brucella Virulence Factor A on the NADH-ubiquinone Oxidoreductase Complex Ⅰ of Mitochondria in Sertoli Cells[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1400-1409.

References

[1] YAN X,HU S,YANG Y,et al.Proteomics investigation of the time course responses of RAW264.7 macrophages to infections with the wild-type and twin-arginine translocation mutant strains of Brucella melitensis[J].Frontiers in Cellular and Infection Microbiology,2021,11(6):679571.
[2] YU J,LI S,WANG L,et al.Pathogenesis of Brucella epididymoorchitis-game of Brucella death[J].Critical Reviews in Microbiology,2022,48(1):96-120.
[3] NAZ H,KORKMAZ P,CEVIK F,et al.The clinical and laboratory characteristics,treatments,and outcomes of patients with Brucella epididymoorchitis (BEO) compared to those without BEO[J]. Turkish Journal of Medical Sciences,2016,46(5):1323-1328.
[4] MKSKYTTASEON S.Brucella epididymoorchitis initially mimicking choledocholithiasis[J].Infection & Immunity,2011,39(1):393-394.
[5] MATHUR S,BANAI M,COHEN D.Natural Brucella melitensis infection and Rev.1 vaccination induce specific Brucella O-polysaccharide antibodies involved in complement mediated Brucella cell killing[J].Vaccines(Basel),2022,10(2):317.
[6] LAVIGNE J P,PATEY G,SANGARI F J, et al.Identification of a new virulence factor,BvfA,in Brucella suis[J].Infection and Immunity,2005,73(9):5524-5529.
[7] 贾芳,王鑫,王玉炯,等.bvfA基因对布鲁氏菌S19株生物学特性及睾丸支持细胞影响的研究[J].中国预防兽医学报,2022,44(6):611-616. JIA F,WANG X,WANG Y J,et al.Influence of bvfA gene on the biological characteristics of B.abortus S19 strain and Sertoli cells[J].Chinese Journal of Preventive Veterinary Medicine,2022,44(6):611-616.(in Chinese)
[8] LIU X,NIE S,CHEN Y,et al.Effects of 4-nonylphenol isomers on cell receptors and mitogen-activated protein kinase pathway in mouse Sertoli TM4 cells[J].Toxicology,2014,326(9):1-8.
[9] ZHANG Q,XIE X,LIU Y,et al.Fructose as an additional co-metabolite promotes refractory dye degradation:Performance and mechanism[J].Bioresource Technology,2019,46(2):430-440.
[10] WANG X,SUN G,FENG T,et al.Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer’s disease progression[J].Cell Research,2019,29(6):787-803.
[11] LIU H,LIU X,LI X,et al.The molecular regulatory mechanisms of the bacteria involved in serpentine weathering coupled with carbonation[J].Chemical Geology,2021,565:120069.
[12] LAI S,ZHOU H,XIONG W,et al.Changing epidemiology of human brucellosis,China,1955-2014[J].Eemerging Infectious Disease,2017,23(2):184-194.
[13] JIANG H,O’CALLAGHAN D,DING J B.Brucellosis in China:History,progress and challenge[J].Infectious Diseases of Poverty,2020,55(9):55.
[14] TA N,YU R,LIAN H,et al.Analysis of laboratory and serological test results in patients with acute brucellosis during follow-up[J].Journal of Clinical Laboratory Analysis,2022,36:e24205.
[15] BRIDGES H R,FEDOR J G,BLAZA J N,et al.Testicular infection in brucellosis:Report of 34 cases[J].Journal of Microbiology Immunology and Infection,2018,51:82-87.
[16] ZHANG Y,ZHANG Y,ZHONG C,et al.Cr(VI) induces premature senescence through ROS-mediated p53 pathway in L-02 hepatocytes[J].Scientific Reports,2016,6:34578.
[17] BRIDGES H R,FEDOR J G,BLAZA J N,et al.Structure of inhibitor-bound mammalian complex Ⅰ[J].Nature Communications,2020,11:5261.
[18] BAKHTINA A A,PHARAOH G A,CAMPBELL M D,et al.Skeletal muscle mitochondrial interactome remodeling is linked to functional decline in aged female mice[J].Nature Aging,2023,3(3):313-326.
[19] NUBER F,SCHIMPF J,DI RAGO J P,et al.Biochemical consequences of two clinically relevant ND-gene mutations in Escherichia coli respiratory complex Ⅰ[J].Scientific Reports,2021,11:12641
[20] VERBEKE J,FAYT Y,MARTIN L,et al.Host cell egress of Brucella abortus requires BNIP3L-mediated mitophagy[J].Journal of the European Molecular Biology Society,2023,42:e112817.
[21] LOBET E,WILLEMART K,NINANE N,et al.Mitochondrial fragmentation affects neither the sensitivity to TNFalpha-induced apoptosis of Brucella-infected cells nor the intracellular replication of the bacteria[J].Scientific Reports,2018,8:5173.
[22] HE Y,REICHOW S,RAMAMOORTHY S,et al.Brucella melitensis triggers time-dependent modulation of apoptosis and down-regulation of mitochondrion-associated gene expression in mouse macrophages[J].Infection & Immunity,2006,74:5035-5046.
[23] MICHAEL O,EZE L Y,ROBERT M,et al.Effects of opsonization and gamma interferon on growth of Brucella melitensis 16M in mouse peritoneal macrophages in vitro[J].Infection and Immunity,2000,74:257-263.
[24] JONES S M,WINTER A J.Survival of virulent and attenuated strains of Brucella abortus in normal and gamma interferon-activated murine peritoneal macrophages[J].Infection and Immunity,1992,60:3011-3014.
[25] CHEN X,LIU H,SUN W,et al.Elevated urine histone 4 levels in women with ovarian endometriosis revealed by discovery and parallel reaction monitoring proteomics[J].Journal of Proteomics,2019,204:103398.
[26] JAIN A P,SAMBATH J,SATHE G,et al.Pan-cancer quantitation of epithelial-mesenchymal transition dynamics using parallel reaction monitoring-based targeted proteomics approach[J].Journal of Translational Medicine,2022,20(1):84.

Multi-omics Analysis of the Effect of Brucella Virulence Factor A on the NADH-ubiquinone Oxidoreductase Complex Ⅰ of Mitochondria in Sertoli Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XU Zhenyu, XU Jinfeng, DENG Xiaoyu, WANG Yueli, HE Jinke, XIE Shanshan, WANG Zhen, YI Jihai, WANG Yong, MIAO Yuhe, CUI Lijin, CHEN Chuangfu. Bioinformatics Analysis and Immunogenicity Evaluation of Brucella Outer Membrane Vesicles [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2906-2914.
[2]	WEI Chunyan, GUO Jia, ZHU Dexin, ZHANG Wei, ZHU Jiale, DEN Xingmei, JIA Sifeng, LIU Liangbo, ZHANG Hui. Construction of Deletion Strain of Brucella BPE159 Gene and Effect of BPE159 Protein on Expression of Cellular Autophagy Factors [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 26-36.
[3]	DENG Xingmei, CAO Shuzhu, GUO Jia, ZHU Dexin, ZHAO Tianyi, CHAI Yingjin, ZHANG Wei, SHI Chao, JIA Sifeng, ZHANG Hui. Effect of Long Non-coding RNA Gm35082-202 on Cell Pyrodecay Induced by Brucella [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3599-3609.
[4]	XIAO Yangyang, MA Zhongchen, LI Ruirui, CHEN Chuangfu, ZHENG Wei, WANG Yong, WANG Pengyan. Bioinformatic Analysis and Polyclonal Antibody Preparation of Brucella Secreted Protein BspI [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3112-3121.
[5]	QIU Runhui, GUAN Feihu, WANG Zihang, GUO Jia, ZHU Dexin, ZHANG Wei, WEI Chunyan, HUO Mingkai, SUN Zhihua, ZHANG Hui. Construction and Biological Characteristics of Brucella Secreting Protein BMCO Gene Deletion Strain [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1630-1640.
[6]	QIU Runhui, GUAN Feihu, TAO Tingting, LI Jia, ZHAO Tianyi, DENG Xingmei, SHI Chao, SUN Zhihua, ZHANG Hui. Subcellular Localization and Screening of Host Interacting Proteins of Brucella Secreted Protein BPE005 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1438-1448.
[7]	WANG Yueli, SHAO Zhiran, YI Jihai, WANG Yong, WANG Zhen, CHEN Chuangfu. miR-145a-3p Regulates Brucella Induced Mouse Macrophage RAW264.7 Autophagy by Targeting ATG14 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1117-1125.
[8]	XIE Shanshan, YANG Qin, DENG Xiaoyu, YI Jihai, WANG Zhen, CHEN Chuangfu. Effects of Mitochondrial Fusion Protein 2 Gene Interference and Overexpression on Macrophage Apoptosis Induced by Brucella [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 283-293.
[9]	YANG Jiangliu, QIAO Junwei, SONG Qingshan, JIA Fang. Prokaryotic Expression and Bioinformatics Analysis of Virulence Gene BvfA of Brucella S19 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2746-2753.
[10]	YI Minna, Ulaangerel Tseweendolmaa, Toli Davshilt, WANG Xisheng, SHEN Yingchao, DU Ming, REN Hong, MANG Lai, BOU Gerelchimeg. Research Progress on Function,Isolation,Purification and Identification of Sertoli Cells [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2947-2956.
[11]	DAI Guodong, YAN Hongbin, LI Li, FU Baoquan, JIA Wanzhong. Research Progress on Screening Methods for Candidate Vaccine Molecules Against Parasitic Zoonoses [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2177-2187.
[12]	LI Jia, DENG Xingmei, TAO Tingting, ZHU Dexin, QIU Runhui, WANG Zihang, WU Jie, ZHANG Hui. Regulatory Effect of has-miR-5196-3p on Inflammasome During Brucella Infection [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1507-1515.
[13]	SONG Shengnan, GUO Jia, YANG Yicheng, SANG Chunli, YANG Meihua, WANG Yuanzhi. Species Identification of Ticks from Pet Dog and Detection of Brucella Carried by Ticks in Shihezi Area [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1717-1724.
[14]	LI Ruirui, MA Zhongchen, ZHANG Hongyang, WANG Zhen, NUSUFU·Nuersailike, WANG Yong, CHEN Chuangfu. Polyclonal Antibody Preparation and Bioinformatics Analysis of Brucella Secreted Protein BspD [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 676-684.
[15]	YANG Qin, DENG Xiaoyu, ZHAO Xiaoli, ZHANG Huan, XI Jing, YI Jihai, WANG Yong, ZHANG Qian, WANG Zhen, CHEN Chuangfu. Effects of Brucella Type Ⅳ Secretion System on Autophagy,Intracellular Survival and Inflammatory Response of Dendritic Cells [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 257-264.