布鲁氏菌介导的mmu-miR-671-5p的靶基因预测与功能富集性分析

doi:10.16431/j.cnki.1671-7236.2019.10.004

Abstract

Abstract: In order to predict the target gene of mmu-miR-671-5p mediated by Brucella,the differentially expressed mmu-miRNA-671-5p target genes were predicted by miRanda and TargetScan softwares after RAW264.7 cells were infected by Brucella.The predicted target genes were 11 953 and 9 252,respectively.The predicted results of the two softwares were intersected and analyzed by Venn which showed 3 681 overlapping target genes.The functional enrichment were analyzed by GO and KEGG,and then PicTar software was used to further predict the target gene of mmu-miRNA-671-5p.The predicted results were further intersected with the results of Venn which showed that there were 7 target genes was the finally predicted results of mmu-miRNA-671-5p.The relative expression of 7 predictive target genes was verified by Real-time quantitative PCR.The results showed that the relative expression of Tnfrsf1b and Tnip1 genes were extremely significantly decreased (P<0.01).And then the mmu-miRNA-671-5p inhibitors were transfected into RAW264.7 cells and the 7 predictive targets were verified.The results showed that the relative expression of Tnfrsf1b and Tnip1 genes was extremely significantly increased (P<0.01),and the target sites of mmu-miRNA-671-5p and the 3'UTR of Tnfrsf1b and Tnip1 were predicted respectively.The results preliminarily showed that the target genes of mmu-miRNA-671-5p were Tnfrsf1b and Tnip1.Both of them had only one predictive binding site,located at 91 and 305 bp of the full-length positions of 3'UTR of Tnfrsf1b and Tnip1,respectively.This study provided a scientific basis for further revealing the function of mmu-miRNA-671-5p in Brucella infected RAW264.7 cells.

Key words: Brucella; RAW264.7 cells; mmu-miR-671-5p; target gene prediction; functional enrichment analysis

CLC Number:

ZHAO Yu, LUO Yichen, GU Guojing, LI Bowen, LI Wenjie, ZHOU Zhixiong, SHUAI Xuehong, WU Li, CHEN Jixuan, HUANG Qingzhou, JIAO Hanwei. Target Gene Prediction and Functional Enrichment Analysis of mmu-miR-671-5p Mediated by Brucella[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 2843-2850.

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Target Gene Prediction and Functional Enrichment Analysis of mmu-miR-671-5p Mediated by Brucella

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