牦牛Bcl-2、Bax基因克隆及其在生殖轴上的表达分析

doi:10.16431/j.cnki.1671-7236.2019.07.001

Abstract

Abstract:

The aim of this study was to clarify CDS sequences and expression characteristics of Bcl-2 and Bax genes in the reproductive axis of female yak,in order to provide theoretical basis for investigating the potential role of Bcl-2 and Bax in regulation of yak reproduction.The samples of hypothalamus,pituitary,ovary,oviduct and uterus of female yak and cattle were collected.The yak Bcl-2 and Bax genes were amplified,cloned and analyzed by RT-PCR and bioinformatics methods.Their mRNA expression levels in different tissues were detected by Real-time PCR.The results showed that the Bcl-2 gene coding region of yak was 690 bp,encoding 229 amino acid.The homology of Bcl-2 gene in yak with Bos taurus was the highest (99.86%),and followed by Capra hircus (98.41%) and Ovis aries (97.97%).The phylogenetic tree showed that the closest genetic relationship was found between yak and Bos taurus.The Bax gene coding region of yak was 579 bp,encoding 192 amino acids.The homology of Bax gene in yak with Bos taurus,Tibetan goat and Jintang Black goat were higher,which was 99.83%,99.48% and 99.48%,respectively,and followed by Ovis aries (99.14%),Equus caballus (95.34%) and Homo sapiens (94.30%).The phylogenetic tree showed that the closest genetic relationship was found between yak and Bos taurus.There were no signal peptides in Bcl-2 and Bax proteins,all of which were acidic unstable hydrophobins.Real-time PCR results showed that Bcl-2 and Bax genes were expressed in hypothalamus,pituitary,ovary,oviduct and uterus tissues of yak and cattle.The expression of Bcl-2 gene was significantly higher in yak ovary (P<0.05) and uterus (P<0.01) than that in cattle.The expression of Bax in yak uterus and ovidut was significantly higher than that in cattle (P<0.05).The Bcl-2/Bax ratio in the yak ovary was extremely significantly higher than that in cattle (P<0.01),and that in uterus and pituitary were significantly higher than cattle (P<0.05).These results indicated that Bcl-2 and Bax were relatively conservative in animal evolution.Bcl-2 and Bax played an important role in reproductive activities.The high expression level of them in yak ovary,uterus,oviduct and pituitary might be related to the anti-apoptotic effect of yak in extreme environment.

Key words: yak; cattle; Bcl-2 gene; Bax gene; cloning; tissue expression

CLC Number:

S823.8^＋5

XIA Yi, WANG Qin, HE Xiangdong, CHEN Ying, JIGE Moti, ZI Xiangdong. Cloning and Expression Analysis of Bcl-2 and Bax Genes in Reproductive Axis of Yaks[J]. , 2019, 46(7): 1881-1889.

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Cloning and Expression Analysis of Bcl-2 and Bax Genes in Reproductive Axis of Yaks

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