不同添加水平多聚甲醛对绵羊瘤胃微生物数量及消化酶活性的影响

doi:10.16431/j.cnki.1671-7236.2019.03.010

Abstract

Abstract:

Five male sheep with the body weight of (55.6±3.2) kg,2 to 3 years old,fitted with permanent rumen fistula and 5×5 Latin square design were used in this experiment to study the effects of different supplemental doses of paraformaldehyde on the microbiocoenosis and activities of digestive enzymes in rumen of sheep.The sheep was fed with the same base diet supplemented with 0,150,300,450 and 600 mg/kg paraformaldehyde,respectively.The pH and concentrations of the ammonia nitrogen and volatile fatty acids,the activities of the endocellulase,exocellulase,xylanase,pectinase,cellobiase,amylase and protease activities,the numbers of bacteria and protozoa,DNA copies of fungi in rumen fluid were determined.The results showed that,compared to control group,the groups supplemented with 150,300,450 and 600 mg/kg paraformaldehyde respectively,the voluntary intakes of dry matter were increased by 2.3% (P>0.05),22.1%(P<0.01),7.5% (P<0.01) and -6.6% (P<0.01) respectively;The protozoa numbers in rumen fluid were decreased by 14.2%,62.5%,72.6% and 85.3% (P<0.01) respectively;And the total numbers of bacteria increased by 35.8%,93.8%,-6.8% and -24.2% (P < 0.01),respectively;The copies numbers of fungi were increased by 2.0% (P>0.05),5.4% (P>0.05),14.3% (P<0.01) and 26.8% (P<0.01),respectively;The ammonia N of rumen fluid were decreased by 9.0%,12.0%,14.9% and 20.9% (P<0.01),respectively;The volatile fatty acids were increased by 2.4% (P>0.05),-0.3% (P>0.05),-1.3% (P>0.05) and -10.3% (P<0.01),respectively;The activities of endocellulase were increased by 6.5%,22.4%,-10.2% and -19.2% (P<0.01),respectively;The activities of exocellulase were increased by 3.4% (P>0.05),17.5% (P<0.01),-18.0% (P<0.01) and -25.7% (P<0.01),respectively;The activities of cellobiase were increased by 8.3%,23.7%,-24.9% and -31.8% (P<0.01),respectively;The activities of amylase were increased by 2.8%,8.6%,16.0% and 23.3% (P<0.01),respectively;The activities of pectinase were decreased by 11.8%,12.1%,11.2% and 11.1% (P<0.01),respectively;The activities of protease were decreased by 10.9%,30.4%,49.6% and 56.2% (P<0.01) respectively;However,the activities of xylanase did not show any significant changes (P>0.05).It was concluded that when the supplemental doses of paraformaldehyde was 300 mg/kg,the voluntary intake of sheep was the highest,and the protozoa number in rumen fluid was decreased,the total number of bacteria and the cellulase activity were the highest.

Key words: paraformaldehyde; sheep; rumen; microorganism quantity; digestive enzyme

CLC Number:

S816.7

WANG Xiaodong, LUO Qiujiang, ZANG Changjiang, XIE Jiaxun, CAI Juan. Effects of Different Supplemental Doses of Paraformaldehyde on the Microorganism Quantity and Activities of Digestive Enzymes in the Rumen of Sheep[J]. , 2019, 46(3): 719-731.

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Effects of Different Supplemental Doses of Paraformaldehyde on the Microorganism Quantity and Activities of Digestive Enzymes in the Rumen of Sheep

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