欣华鸡DRD2基因多态性及其与蛋用性状的关联分析

doi:10.16431/j.cnki.1671-7236.2019.02.016

Abstract

Abstract:

This study was aimed to investigate the polymorphism of dopamine receptor D2 (DRD2) gene and its correlation with egg traits in Xinhua chicken,and find the molecular genetic markers for the breeding of egg traits in Xinhua chicken.4 pairs of primers were designed using Primer Premier 5.0,the genotype of 473 individuals in Xinhua E-line chicken were identified by PCR-RFLP method,the correlation were analyzed between egg traits of Xinhua chicken and polymorphism of DRD2 gene using SPSS 19.0 software.Population polymorphism analysis results showed that there were 4 SNPs of A-16105G (SNP1),G-12510T (SNP2),G+3360A(SNP3) and T+5042C(SNP4),SNP1 and SNP2 were in Hardy-Weinberg equilibrium (P>0.05),and the heterozygosity were low,SNP3 and SNP4 significantly deviated from the Hardy-Weinberg equilibrium (P<0.05).Correlation analysis results showed that 4 SNPs of DRD2 gene were associated with egg traits in Xinhua E-line chicken,for egg production traits,SNP1 was significantly associated with age at the first egg (P<0.05);SNP2 was extremely significantly associated with egg production at 33 weeks old and the total number of eggs at 300 days of age (P<0.01);SNP3 was associated with body weight at sexual maturity (P<0.05),the total number of eggs at 300 days of age (P<0.01),average clucth size (P<0.01) and the largest clucth size (P<0.05);SNP4 was extremely significantly associated with age at the first egg (P<0.01).For egg quality traits,SNP1 was associated with egg-shaped index (P<0.01),yolk color (P<0.01) and Haugh units (P<0.05);SNP2 was associated with yolk weight (P<0.05);SNP3 was extremely significantly associated with eggshell strength (P<0.01);SNP4 was significantly associated with yolk weight,albumen weight and Haugh units (P<0.05).The haplotype analysis results showed that the different haplotype combinations of 4 SNPs of DRD2 gene were significantly correlated with the longest clutch size in chicken (P<0.01) and haugh units of eggs (P<0.05).The tissue expression profiling results indicated that DRD2 gene was mainly expressed of pituitary gland in Xinhua chicken,and was also highly expressed in chest muscle at 58 weeks old,with a low expression in brain at 36 weeks old.The results suggested that DRD2 gene in Xinhua E-line chicken could be used to assist the genetic improvement of egg traits.

Key words: Xinhua chicken; DRD2 gene; egg production traits; egg quality

CLC Number:

S813.3

QU Ke, HUANG Tao, GONG Yanzhang. Polymorphism of DRD2 Gene and Its Associations with Egg Traits in Xinhua Chicken[J]. , 2019, 46(2): 458-469.

References

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Polymorphism of DRD2 Gene and Its Associations with Egg Traits in Xinhua Chicken

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