›› 2018, Vol. 45 ›› Issue (7): 1873-1880.doi: 10.16431/j.cnki.1671-7236.2018.07.018

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Study on the Innate Immunity of Local and Introduced Breeds in Qinghai Province

ZHANG Yingyue1, WANG Wenqiang1, MA Liqing2, PU Yabin1, MA Yuehui1, ZHAO Qianjun1   

  1. 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. Qinghai Academy of Animal Science and Veterinary Medicine, Xining 810016, China
  • Received:2017-11-08 Online:2018-07-20 Published:2018-07-20

Abstract:

To explore the innate immunization level and breed characteristics of Qinghai local and introduced breeds,186 individuals of two local breeds (Euler and White Tibetan sheep) and one introduced breed (Poll Dorset sheep) were chose,and fresh blood samples were collected for routine blood test (WBC,RBC,HGB,HCT,MCV,MCH,MCHC,PLT,RDW-CV and LYM%);Serum was prepared to detect parameters of IL-1,IL-2,IL-6,IL-18,IFN-γ,IgG and MHC using ELISA-Kit,the comparative results were analyzed using different breed and breed origin as dependent variables.When breed was used as the dependent variable,Poll Dorset sheep contained the highest immune level and the disease resistance of White Tibetan sheep was slightly higher than that of Euler sheep.When breed origin was used as the dependent variable,the analysis results showed that the innate immunity level of local breeds was lower than that of introduced breed,the diseases resistance was weak.The blood biochemical level of Poll Dorset sheep was higher than local sheep except for MCH,MCHC and RDW-CV.This results provided reference data for the study of innate immunity of different sheep breeds,and had guiding for breeding and breed improvement of Tibetan sheep in Qinghai province.

Key words: sheep; innate immunity; ELISA

CLC Number: