蛋鸡脂肪肝综合征模型的建立

doi:10.16431/j.cnki.1671-7236.2018.05.035

Abstract

Abstract:

This study was aimed to establish the fatty liver syndrome model of egg-type chickens.280 five-day-old Hyline egg-type chickens were selected and randomly divided into control group (basic diet group) and model groups (A,B and C groups).From 0 to 10 days of experiment,the model groups was fed with high-fat diet with different ratio,and the routine basal diet was fed from 11 to 20 days.The experimental chicken mental state,appearance and signs,water intake and appetite of chickens were observed and recorded each day.15 chickens were randomly selected from each group on the 0,10th and 20th days of experiment for blood sampling,liver and abdominal fat extraction.The liver coefficient,hepatic lipid ratio,abdominal fat percentage,serum total cholesterol (TC),triglyceride (TG),alanine transaminase (ALT) and aspartate transaminase (AST) levels were determined.The results showed that there was a lot of fat deposits in the abdominal cavity and mesentery in three modle groups on the 10th day of the experiment,the indicators of liver coefficient,hepatic fat percentage,abdominal fat percentage,serum TC,TG,ALT and AST were accord with the diagnostic criteria of fatty liver syndrome model of chickens.On the 20th day of experiment,the levels of ALT and AST were recovered in model groups A and B,and the other indexes were higher than those in control group,the clinical symptoms and the detection indexes in model group C were accord with the diagnostic criteria of fatty liver syndrome model of chickens.Therefore,continuous feeding of high-fat diet C (74.5% basal diet,6% cholesterol,14% lard,5% sucrose,0.5% propylthiouracil) could successfully establish the fatty liver syndrome model of egg-type chickens.

Key words: fatty liver syndrome in egg-type chicken; model establishment; high fat diet; hepatic fat rate; abdominal fat rate

CLC Number:

S831

XING Yujuan, ZHAO Weidan, ZHU Hao, QIN Junjie, HOU Xiaojiao, WANG Xiumin. Establishment of Fatty Liver Syndrome Model of Egg-type Chickens[J]. , 2018, 45(5): 1401-1407.

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Establishment of Fatty Liver Syndrome Model of Egg-type Chickens

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