不同收集方法及添加半胱氨酸、肝素钠对黄牛卵母细胞体外成熟及发育的影响

doi:10.16431/j.cnki.1671-7236.2018.05.022

Abstract

Abstract:

This study was aimed to examine the effects of different collection methods and supplementation of heparin and cysteine sodium on in vitro maturation and fertilization of bovine oocytes.In this experiment,bovine oocytes obtained from ovarian follicles were collected by two methods including the cutting and ovulation methods which were made comparison.The results showed that the mature rate of collected oocyte by ovulation method was significantly higher than cutting method (P<0.05).Furthermore,collected oocytes were divided into 4 groups:Group A (control group,maturation culture medium),group B (maturation culture medium+200 μmol/L cysteine),group C (maturation culture medium+20 μg/mL heparin sodium) and group D (maturation culture medium+200 μmol/L cysteine+20 μg/mL heparin sodium).The results showed that the maturation rate of oocytes in group D was significantly higher that groups A,B and C (P<0.05),there was no significant difference between groups B and C (P>0.05),but both of them were significantly higher than group A (P<0.05).The cleavage rate of oocytes in group A was significantly lower than groups B,C and D (P<0.05),but there was no significant difference in groups B,C and D (P>0.05).The blastocyst rate of group D was significantly higher than other three groups (P<0.05).In conclusion,this study results showed that ovulation method would be the better collecting method,supplementation of heparin sodium and cysteine on oocyte in vitro maturation and fertilization had a promoting effect,and in vitro maturation of adding two substances was better.

Key words: heparin sodium; cysteine; oocytes collection method; in vitro maturation; in vitro fertilization

CLC Number:

S814

XIA Wei, QI Dan, XU Qing, YE Sijie, JIANG Tao. Effects of Different Collection Methods and Supplementation of Heparin and Cysteine Sodium on in vitro Maturation and Fertilization of Bovine Oocytes[J]. , 2018, 45(5): 1297-1303.

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Effects of Different Collection Methods and Supplementation of Heparin and Cysteine Sodium on in vitro Maturation and Fertilization of Bovine Oocytes

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