水泡性口炎病毒N蛋白原核表达及免疫原性分析

doi:10.16431/j.cnki.1671-7236.2017.09.008

Abstract

Abstract:

This study was aimed to clone and express the specific antigen nucleoprotein (N) of vesicular stomatitis virus (VSV), and then purify and analyze its immunogenicity. Based on published VSV genome N gene sequence in GenBank, two kinds of N genes of VSV with different serotypes were synthesized, respectively. After sequence analysis, one pair of specific primers was designed and synthesized, N gene fragment with about 1 300 bp length was amplified by PCR, and subcloned into pCold Ⅰ expression vector. The recombinant N protein was induced with IPTG and purified by Ni-NTA. The results of SDS-PAGE showed that the N gene was successfully expressed in E. coli and the molecular weight of protein was 50 ku; The results of Western blotting showed that this recombined protein specifically reacted with polyclonal antibody serum of VSV. The recombinant vector with VSV-IND and VSV-NJ were successfully constructed, and the N protein was solubly expressed in E. coli, and the purified protein demonstrated promising immunogenicity.

Key words: vesicular stomatitis virus (VSV); N protein; prokaryotic expression

CLC Number:

S852.65

ZHANG Xue, LOU Li, CHEN Yang, ZHOU Xiao-li, LIU Zhao, JIA Yun, SUN Ying-jie. Prokaryotic Expression and Immunogenicity Analysis of N Protein from Vesicular Stomatitis Virus[J]. , 2017, 44(9): 2593-2597.

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Prokaryotic Expression and Immunogenicity Analysis of N Protein from Vesicular Stomatitis Virus

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