广东新型鸭呼肠孤病毒σB蛋白基因克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2017.02.004

Abstract

Abstract:

In order to study the genetic variation and evolution of new-type duck reovirus (NDRV) in Guangdong province, some samples (liver and spleen) were collected from different sick ducks in Guangdong province. Eight strains were initially isolated and identified. σB protein genes were amplified, cloned and sequenced by RT-PCR method,and σB protein characteristics of amino acid, antigen and genetic analysis of system evolution were compared with other strains. The results showed that the isolated viruses were the NDRV. Comparison of nucleotide sequences showed the NDRV isolated from duck in Guangdong province were more closely related to DRV reported in other parts of the country, the homology reached 96.6% to 99.5%, the NDRV isolated from duck in Guangdong province were not closely related with ARV and MDRV, the homology was 64.6% to 66.5% and 66.2% to 67.1%, respectively. Homology comparison of nucleotide between 8 strains NDRV were 97.7% to 99.7%.Compared with other strains, NDRV phosphorylation sites were less than ARV and MDRV reference strains, and the most area of the antigen index were high, similar to MDRV. Genetic evolution analysis results showed that NDRV and other domestic DRV were in the evolution of the same branch, but ARV and MDRV were in different branches. The result showed σB protein gene sequences of the NDRV were highly conserved,and NDRV isolated from Guangdong province had no significant regional differences with other regions.

Key words: new-type duck reovirus; σB protein; cloning; sequence analysis

CLC Number:

S852.65⁺9.4

HUANG Wen-jing, LIANG Guo-zhi, MEI Min-min, LI Xiao-wen, HUANG Shu-jian. Cloning and Sequence Analysis of σB Protein Genes of New-type Duck Reovirus Isolated from Guangdong[J]. , 2017, 44(2): 327-335.

References

[1] 陈少莺,陈仕龙,林锋强,等. 新型鸭呼肠孤病毒的分离与鉴定[J]. 病毒学报,2012,28(3):224-230.
[2] Wang D, Xu F, Ma G M, et al. Complete genomic sequence of a new -origin reovirus from China[J]. J Virol, 2012, 86(22): 12445.
[3] 陆新浩,陈秋英,刘鸿,等. 宁波地区新型鸭呼肠孤病毒病的流行情况[J]. 中国家禽,2015,37(4):58-59.
[4] 黄瑜, 傅光华, 施少华, 等. 新致病型鸭呼肠孤病毒的分离鉴定[J]. 中国兽医杂志, 2009,45(12):29-31.
[5] 陈宗艳, 朱英奇, 王世传, 等. 一株新型鸭源呼肠孤病毒(TH11株)的分离与鉴定[J]. 中国动物传染病学报, 2012,20(1):10-15.
[6] 袁远华, 王俊峰, 吴志新, 等. 1株番鸭源新型鸭呼肠孤病毒(QY株)生物学鉴定[J]. 中国兽医学报, 2013,33(8):1174-1178.
[7] Le Gall-Recule G, Cherbonnel M, Arnauld C, et al. Molecular characterization and expression of the S3 gene of reovirus 89026[J]. J Gen Virol, 1999,80(Pt 1):195-203.
[8] Kuntz-simon G,Le Gall-Recule G,De Boisseson C,et al.Muscovy duck reovirus σC protein is a typically encoded by the smallest genome segment[J]. J Gen Virol,2002,83(5):1189-1200.
[9] Zhang Y,Guo D,Liu M,et al.Characterization of the σB-encoding genes of reovirus:σC-σB-ELISA forantibodies against duck reovirus in ducks[J]. Vet Microbiol,2007,9(3):231-241.
[10] 刘美荣. 一例雏半番鸭疑似新型鸭呼肠孤病毒病的诊治[J]. 畜禽业, 2012,278(6):76-77.
[11] 朱英奇.新型鸭呼肠孤病毒的鉴定及其全基因组序列解析[D].合肥:安徽农业大学,2013.
[12] Yun T, Yu B, Ni Z, et al. Genomic characteristics of a novel reovirus from ling in China[J]. Vet Microbiol,2014,171(1-2):261-271.
[13] 王丹,张大丙. 水禽源呼肠孤病毒的全序列测定和分型[A]. 中国畜牧兽医学会禽病学分会.第四届全国禽病分子生物技术青年工作者会议论文集[C].2015.
[14] 杨志刚. 共表达禽(番鸭)呼肠孤病毒σB-σC-LTB蛋白亚单位疫苗载体的构建及免疫保护的研究[D].北京:中国农业科学院,2014.
[15] 陈祥,吴海洋,王永强,等. 禽呼肠孤病毒σB蛋白单克隆抗体的制备及鉴定[J]. 中国兽医杂志,2015,51(6):3-6.
[16] 谢志勤,谢芝勋,刘加波,等.禽呼肠孤病毒S1733株单克隆抗体的制备及特性鉴定[J].中国畜牧兽医,2012,39(11):47-51.
[17] 汪军卿. 新城疫病毒P基因RNA编辑及P蛋白磷酸化位点对病毒复制的影响[D].北京:中国农业科学院,2014.
[18] 韩典霖. 禽(番鸭)呼肠孤病毒YB株S3与S4 ORF2基因的表达、纯化及免疫原性研究[D]. 福州:福建农林大学, 2007.
[19] 张云, 陈小丹, 李永峰, 等. 抗番鸭呼肠孤病毒σB蛋白单克隆的制备和鉴定[A].中国畜牧兽医学会兽医公共卫生学分会第二次学术研讨会论文集[C]. 2010

Cloning and Sequence Analysis of σB Protein Genes of New-type Duck Reovirus Isolated from Guangdong

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[2]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[3]	ZHANG Yongjie, SUI Zhiyuan, ZHANG Zhishuai, WANG Chenguang, LI Xiaojun, XING Feng. Cloning and Bioinformatics Analysis of NPTX1 Gene and Its Tissues Expression at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1048-1058.
[4]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[5]	TANG Jingyu, DU Hanyu, MENG Chunchun, LIU Guangqing. Cloning,Bioinformatics Analysis and Subcellular Localization of Interferon-stimulated Gene 15 in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2843-2854.
[6]	QI Nannan, QIAN Yonghang, LUO Gang, SITU Xuqi, LIU Jiying. Cloning, Bioinformatics Analysis and Tissue Expression Location of Porcine MORC2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2431-2441.
[7]	ZHANG Hang, SHA Huiyang, LI Huawei, HUANG Liangzong, ZHAO Mengmeng. Cloning, Bioinformatics and Tissue Expression Analysis of TRIM3 Gene in Swine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2474-2483.
[8]	YAN Yelian, ZHANG Mengya, LIU Qiuchen, WANG Xin, XU Changzhi, ZONG Yanfeng, ZHU Zhihua, WU Sucheng, SONG Yu, LI Yunsheng, ZHANG Yunhai, CAO Zubing. Research Progress of Epigenetic Reprogramming in Mammalian Somatic Cell Nuclear Transfer Embryo Development [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2259-2269.
[9]	WANG Baobao, JIN Hang, XIAN Yuhan, FENG Hongsheng, GAO Fengshan. Cloning SLA-1 Gene from Porcine Kidney Epithelial Cells and Analyzing its Molecular Structure Characteristics [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1671-1678.
[10]	ZHANG Jihu, MI Renisa, WANG Zixuan, LI Lin, XING Feng, LIU Shudong. Cloning and Bioinformatics of ERα Gene and Its Expression of Ovary Tissue in Cele Black Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1679-1687.
[11]	SHEN Xiang, CHEN Linghui, YAO Jingjie, WANG Sanhu, BAI Yueyu, ZHANG Xiaojian. Cloning,Bioinformatics and Expression Analysis of Bovine CCR7 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1244-1252.
[12]	ZHANG Zhishuai, SUI Zhiyuan, ZHANG Jihu, LI Qingjin, XING Feng. Cloning of CNP Gene and Its Tissue Expression Characteristics at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1402-1412.
[13]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[14]	HOU Jinxing, WANG Zhanhang, ZHU Junru, JIANG Yue, LIU Shujuan, AN Xiaopeng. Cloning, Bioinformatics and Tissue Expression Profile Analysis of CSN1S1 Gene in Guanzhong Dairy Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 857-865.
[15]	WEI Yunfang, LI Feixiang, Xing Yun, Huang Qingguo, LI Judong, WAN Jiusheng, CHEN Fangliang. Study on Cloning, Bioinformatics Analysis and Spatio-temporal Expression of IGF2 Gene in Kunming Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 866-875.