黄藤素对山羊子宫内膜上皮细胞的体外毒性研究

doi:10.16431/j.cnki.1671-7236.2016.12.023

Abstract

Abstract:

To explore the cytotoxicity of palmatine in vitro,goat endometrial epithelial cells (EECs) were stimulated by different concentrations of palmatine,then the growth curve of cell was drew by MTT assay which could detect the cell proliferation. The cell growth state was observed by inverted microscope,and Wright-Giemsa staining was used to observe morphological changes of the cells while LDH test was used to check the membrane permeability of EECs. The results showed that when the concentration was 10 to 227 μg/mL,palmatine had a role in promoting proliferation of the EECs,while when the concentration was more than 227 μg/mL,cell proliferation was inhibited,and 50% inhibitory rate of proliferation (IC₅₀) of this drug on EECs was 360 μg/mL.Wright-Giemsa staining results showed that themorphology of the nucleus and cytolymph were not significantly changed when 100 and 200 μg/mL palmatine effected EECs. LDH results showed that when the palmatine concentration was more than 250 μg/mL,LDH content was extremely significantly higher than that of normal cell group(P<0.01).In conclusion,palmatine presented certain toxic effect on EECs with a significant dose-response effect,and the safe dose range of palmatine was less than 250 μg/mL.

Key words: palmatine; endometrial epithelial cells; cytotoxicity; membrane permeability

CLC Number:

R282.71

YAN Bao-qi, DONG Shu-wei, WANG Dong-sheng, NA Li-dong, SANG Meng-qi, YANG Hong-zao, ZHANG Shi-dong, YAN Zuo-ting. The Study on the Cytotoxicity of Palmatine on Goat Endometrial Epithelial Cells in vitro[J]. , 2016, 43(12): 3239-3244.

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The Study on the Cytotoxicity of Palmatine on Goat Endometrial Epithelial Cells in vitro

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