沙子岭猪ASB17基因克隆、生物信息学分析及组织差异表达研究

doi:10.16431/j.cnki.1671-7236.2016.11.002

Abstract

Abstract:

In this study,ASB17 gene cDNA full length in Shaziling pig was cloned by RACE and it was analyzed by bioinformatics,the ASB17 gene expression levels were detected in Shaziling pig D30 period of nine different tissues（testis,muscle,intestine,lung,heart,kidney,liver,spleen,fat）and eight different developmental stages（E90,D1,D30,D60,D90,D120,D150,D180）of testicular tissue by Real-time quantitative PCR.The results showed that the length of ASB17 gene cDNA was 1 135 bp,and the ORF region was 888 bp,which encoded 295 amino acids.Real-time quantitative PCR results showed that the D30 period's expression level of ASB17 gene mRNA was the highest in testis,followed by fat and spleen,while the expression of ASB17 gene mRNA in muscle and small intestine tissue were the lowest,there were significant difference in testis and muscle,small intestine,lung（P<0.05);In the testis,the expression of ASB17 gene mRNA was the highest in D120 and D150 periods,while slightly declined in D180,the differences were extremely significant beween E90,D1,D30,D60 and D90,D120,D150,D180（P<0.01);The differences were not significant beween D90 and D180（P>0.05),while there were extremely significant with other periods（P<0.01);The sexual maturity periods of D120,D150,D180 had no significant differences（P>0.05).The full length of ASB17 cDNA was cloned and the expression levels of ASB17 gene in different tissues and different developmental stages were detected in this study,which laid the foundation for further research on the function of ASB17 gene.

Key words: Shaziling pig; ASB17 gene; cloning; gene expression

CLC Number:

DONG Lian-hua, RAN Mao-liang, WENG Bo, LI Zhi, PENG Fu-zhi, CHEN Bin. Cloning,Bioinfornatics and Tissue Differential Expression Analysis of ASB17 Gene in Shaziling Pig[J]. , 2016, 43(11): 2811-2819.

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Cloning,Bioinfornatics and Tissue Differential Expression Analysis of ASB17 Gene in Shaziling Pig

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