猪传染性胃肠炎病毒截短S基因原核表达及免疫原性初步检测

doi:10.16431/j.cnki.1671-7236.2016.10.005

Abstract

Abstract:

To monitor TGEV antibody in pig,major antigenic fragments of TGE B/C zone was amplified by PCR,purified PCR product and pET-32a which were separately digested,and were connected and transformed into the BL21 competent cells.We picked out successfully transformed bacterial colonies to amplify by PCR,and put the remained bacterial colonies into LB solution to culture overnight,which was extracted to get recombinant plasmid and sequenced.We cultivated right sequenced bacteria to induce protein to express by IPTG,there was a right target band in 37 ku,which was purified by cutting polyacrylamide gel,then detected by Western blotting with homemade anti-rabbit serum,specific band (about 37 ku) could finally be seen in the right position,which proved that this recombinant protein was immunogenic.It would profitably create ELISA-kit to supervise TGEV antibody,and effectively provide some relevant value in immune situation of pig.

Key words: TGEV; B/C zone; truncated expression

CLC Number:

ZHENG Zhong-hua, YANG Pei, ZHAO Ming, QIU Feng, WU Zu-qing, ZHANG Shi-yun, LIU Qiao-fan, HE Hong-zhou, HONG Chun-dan, WEI Chu-dan, ZHAO Shuang, LI Pan-pan, CHEN Jin-ding. Prokaryotic Expression of Truncated S Gene of Porcine Transmissible Gastroenteritis Virus and Preliminary Test of Immunogenicity[J]. , 2016, 43(10): 2541-2546.

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Prokaryotic Expression of Truncated S Gene of Porcine Transmissible Gastroenteritis Virus and Preliminary Test of Immunogenicity

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