水貂细小病毒NS1与VP2蛋白的原核表达及免疫原性分析

doi:10.16431/j.cnki.1671-7236.2016.06.035

Abstract

Abstract: In order to develop subunit vaccine of mink enteritis virus,the immunogenicity of mink parvovirus protein NS1 and VP2 had been evaluated.Two pairs of primers were designed,and the full-length NS1 and VP2 genes had been amplificated,and then prokaryotic expression vector pET-32a-NS1,PET-32a-VP2 were constructed.After the analysis of SDS-PAGE and Western blotting,target proteins had been purified by His-Bind affinity chromatography.The immunogenicity of purified protein NS1 and VP2 were evaluated by serum ELISA testing,after inoculated BALB/c mouse.The results showed that the molecular mass of NS1 and VP2 protein were 83 and 67 ku by SDS-PAGF and Western blotting;Although both target protein NS1 and VP2 had the ability to induce BALB/c mouse to produce anti-MEV specific antibodies,the level of antibodies induced by the protein VP2 was higher than protein NS1.Mink parvovirus protein VP2 was more suitable for the development of subunit vaccine.

Key words: mink parvovirus; prokaryotic expression; recombinant protein

CLC Number:

S852.65⁺9.2

WANG Yang, NIU Deng-yun, LIU Hao, HU Bo, MA Fan-shu, LU Rong-guang, LV Shuang, YAN Xi-jun. Prokaryotic Expression and Immunogenicity Analysis of Mink Parvovirus NS1 and VP2 Protein[J]. , 2016, 43(6): 1630-1634.

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Prokaryotic Expression and Immunogenicity Analysis of Mink Parvovirus NS1 and VP2 Protein

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