紫菀乙醇提取物对豚鼠离体气管平滑肌收缩功能的影响

doi:10.16431/j.cnki.1671-7236.2016.06.026

Abstract

Abstract: The aim of the experiment was to study the effect of alcohol extract of Aster tataricus L.f.on the contraction of isolated guinea pig tracheal smooth muscle.The guinea pig tracheal smooth muscle was prepared by isolated tracheal thermostatic perfusion method.The effects of Aster tataricus L.f.on tracheal smooth muscle under the resting state and contraction stimulated by acetylcholine (Ach),histamine (His),CaCl₂,Ca²⁺ release and influx in cells without calcium were measured by BL-420F biological function experiment systems.The results showed that Aster tataricus L.f.could induce the contraction of isolated trachea at resting state at a low concentrations of 0.002 to 0.008 g/mL and exerted a relaxation on the isolated trachea when at the higher concentrations of 0.008 to 0.196 g/mL.All of the three concentrations of Aster tataricus L.f.could inhibit the tension stimulated by Ach,His and CaCl₂ which were antispasmodic agents,led to the non-parallelled rightward moving of cumulative concentration-response curve and the decline of maximal responses.The suppressive effect on His was the strongest,followed by Ach and CaCl₂,and the suppression could counteract the contraction induced by extracellular Ca²⁺influx significantly,and they were all expressed concentration-dependent manner.These results indicated the alcohol extract of Aster tataricus L.f.could relax tracheal smooth muscle by acting on M-receptor and histamine receptor and blocking Ca²⁺ passage,thus inhibiting extracellular Ca²⁺influx.

Key words: alcohol extract of Aster tataricus L.f.; guinea pig; tracheal smooth muscle; changes of tension

CLC Number:

S853.74

PENG Wen-jing, XIN Rui-hua, LIU Yan, LUO Yong-jiang, WANG Gui-bo, LUO Chao-ying, XIE Jia-sheng, LI Jin-yu, ZHENG Ji-fang. Effects of Alcohol Extract of Aster tataricus L.f.on the Contraction of Guinea Pig Tracheal Smooth Muscle in vitro[J]. , 2016, 43(6): 1572-1578.

References

[1] 王国艳,吴弢,林平川,等.紫菀三萜类化学成分的研究[J].中草药,2003,34(10):875-876.
[2] 刘可越,张铁军,高文远,等.紫菀中多酚类化合物的研究[J].中草药,2007,38(12):1793-1795.
[3] 卢艳花,王峥涛,徐珞珊,等.紫菀中的多元酚类化合物[J].中草药,2002,33(1):17-18.
[4] 刘可越,张铁军,高文远,等.菀中三萜及甾体化合物的研究[J].天然产物研究与开发,2006,18(1):4-6.
[5] 卢菲,任晓倩,张朝凤,等.紫菀正丁醇萃取液化学成分研究[J].亚太传统医药,2013,9(8):42-43.
[6] 卢艳花,戴击,王峥涛,等.紫莞祛痰镇咳作用及其有效部位和有效成分[J].中草药,1990,30(5):360-362.
[7] 彭文静,辛蕊华,任丽花,等.紫菀化学成分及药理作用研究进展[J].动物医学进展,2015,36(3):102-107.
[8] Morita H,Nagashima S,Uchiumi Y,et al.Cyclic peptides from higher olants.XXV Ⅲ.Antitumor activity and hepatic microsomal biotransformation of cyclic pentapeptides,astins,from Aster tataricus[J].Chem Pharm Bull,1996,44(5):1026-1032.
[9] Zhang Y X,Wang Q S,Wang T,et al.Inhibition of human gastric ccarcinoma cell growth in vitro by a polysaccharide from Aster tataricus[J].Int J Biol Macromol,2012,51(4):509-513.
[10] Du L,Mei H F,Yin X,et al.Delayed growth of glioma by a polysaccharide from Aster tataricus involve upregulation of Bax/Bcl-2 ratio,activation of caspase-3/8/9,and downregulation of the Akt[J].Tumor Biol,2014,35(3):1819-1825.
[11] 杨滨,肖永庆,梁日欣,等.紫菀挥发油中祛痰活性化学成分研究[J].中国中药杂志,2008,33(3):281-283.
[12] 张建伟,窦昌贵,张勉,等.紫菀、款冬及其配伍的毒性及药效学研究[J].中国临床药理学与治疗学,2007,12(4):405-411.
[13] 张巧真,张燕,张勉,等.款冬花止咳有效部位和紫菀配伍"相须"部位的研究[J].时珍国医国药,2009,20(5):1042-1044.
[14] 刘令勉,于仲范,吴翠玉.紫菀散加甘草对离体豚鼠气管解痉作用的研究[J].中国中药杂志,1993,18(9):566-567.
[15] 裴昆,夏放高,陈海芳,等.柚皮苷对豚鼠离体气管平滑肌作用的机制研究[J].中药新药与临床药理,2015,26(1):17-21.
[16] 唐春萍,江涛,陈志燕.野马追对豚鼠离体气管平滑肌收缩功能的影响[J].中药药理与临床,2002,18(6):30-32.
[17] 魏莹,裴昆,陈海芳,等.辛弗林对豚鼠离体气管平滑肌的作用及机制[J].中国实验方剂学杂志,2015,21(1):158-162.
[18] 赵益,朱卫丰,刘红宁,等.贝母辛平喘作用及机制研究[J].中草药,2009,40(4):100-104.
[19] 关福兰,金万宝,章新华,等.白花前胡甲素对高钾、乙酰胆碱预收缩的离体家兔气管平滑肌的作用[J].中国医科大学学报,1994,23(6):549-552.
[20] 何冰,陈小夏,王经韵.白藜芦醇对兔离体气管平滑肌收缩功能的影响[J].中国中医基础医学杂志,2010,6:522-523.
[21] 景亮.气道平滑肌:基础与临床[J].临床麻醉学杂志,2001,17(9):522-525.
[22] 赵明沂,朱喆,李智,等.滨蒿内酯对豚鼠离体气管平滑肌作用的研究[J].中国医科大学学报,2000,5:18-20.
[23] 高春艳,聂珍贵,梁翠茵,等.鸭嘴花碱对豚鼠离体气管平滑肌收缩功能的影响[J].天津药学,2003,15(6):4-6.
[24] 韦媛媛,徐峰,陈侠,等.桑白皮总黄酮对豚鼠离体气管平滑肌收缩功能的影响[J].食品科技,2009,34(4):185-187.
[25] Yu P,Cheng S,Xiang J,et al.Expectorant,antitussive,anti-inflammatory activities and compositional analysis of Aster tataricus[J].J Ethnopharmacol,2015,164:328-333.
[26] Ryu J,Lee H J,Park S H,et al.Effects of the root of Platycodon grandiflorum on airway mucin hypersecretion in vivo and platycodin D₃ and deapi-platycodin onproduction and secretion of airway mucin in vitro[J].Phytomedicine,2014,21:529-533.
[27] Ng T B,Liu F,Lu Y H,et al.Antioxidant activity of compounds from the medicinal herb Aster tataricus[J].Comparative Biochemistry and Physiology Part C,2003,136(2):109-115.
[28] 安明胜,孙双勇,安春娜,等.含花青素及甘草酸的中药提取物对豚鼠气管松弛作用的比较[J].中国新药杂志,2013,22(8):945-950.

Effects of Alcohol Extract of Aster tataricus L.f.on the Contraction of Guinea Pig Tracheal Smooth Muscle in vitro

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	ZHENG Hua-bin, ZHANG Zhong-wang, LV Jian-liang, PAN Li, ZHANG Yong-guang. Construction of Co-expression Vector Containing Multi-gene of FMDV and Porcine IFN-α and Its Immunogenicity Effect in Guinea Pig [J]. , 2017, 44(5): 1452-1461.
[2]	XIAO An-ju, YU Xin, YIN Mei-zhen, LU Yan-xiu. Feeding of Hartley Guinea Pigs for Experiment and Establishment of Allergic Rhinitis Model of Nasal Drops [J]. , 2015, 42(5): 1193-1197.
[3]	XING Shou-ye, ZHOU Xu-zheng, LI Bing, NIU Jian-rong, WEI Xiao-juan, ZHANG Ji-yu. Allergic Study of Ivermectin Microemulsion [J]. , 2014, 41(11): 275-278.