猪源抗菌肽PBD-1在毕赤酵母中的表达及鉴定

doi:10.16431/j.cnki.1671-7236.2016.03.012

Abstract

Abstract: In order to produce antimicrobial peptide PBD-1 with bioactivity in Pichia pastoris,according to published amino acid sequence of PBD-1 and the partiality codon of yeast,the PBD-1 gene was amplified by SOE-PCR and cloned into pPIC9K to construct a recombinant expression vector pPIC9K-PBD-1.The recombinant vector was linearized by SacⅠ,and then transformed into SMD1168 by electroporation.Positive yeast expression strain was obtained by PCR.The antimicrobial peptide PBD-1 (approximately 4.5 ku) was expressed by methanol induction.Antibacterial activity assay showed that the antimicrobial peptide PBD-1 had better antibacterial activity against E.coli,S.aureus and B.subtilis.

Key words: PBD-1 gene; expression; Pichia pastoris; PCR

CLC Number:

Q786

JIANG Xue-bin, CHEN Jia-wei, YANG Jun, LIU Shun-zhi, XIAO An-ji, LU Zhi-peng, CHU Pin-pin, HUANG Chao-yuan, CAI Hai-ming, MA Miao-peng, ZHANG Ling-hua. Expression and Identification of Porcine Antimicrobial Peptide PBD-1 in Pichia pastoris[J]. , 2016, 43(3): 644-649.

References

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Expression and Identification of Porcine Antimicrobial Peptide PBD-1 in Pichia pastoris

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