18β-甘草次酸吡啶酰胺衍生物体外抗肿瘤活性研究

doi:10.16431/j.cnki.1671-7236.2015.11.002

Abstract

Abstract: The assay was aimed to study the effect of 18β-glycyrrhetinic acid derivatives containing pyridine carboxamide 6c,10c and 11c on the proliferation of HeLa,SMMC-7721 and SGC-7901 cells.Detected the cellular inhibition rate of 18β-glycyrrhetinic acid derivatives containing pyridine carboxamide 6c,10c and 11c to HeLa,SMMC-7721 and SGC-7901 cells by MTT method;Detected the conditions of apoptosis by Hoechst-33258 Staining Kit;Detected the apoptosis rate by Annexin V-FITC/PI method;The related protein expression levels were detected by Western blotting;To further clarify the situation of apoptosis induced in treatment group by detecting the activity of Caspases-3.18β-glycyrrhetinic acid derivatives containing pyridine carboxamide 6c,10c and 11c had the inhibition and promoting apoptosis effects on the proliferation of HeLa,SMMC-7721 and SGC-7901 cells,and had the concentration dependence.The results all showed statistical significance in t test (P<0.05).18β-glycyrrhetinic acid derivatives containing pyridine carboxamide 6c,10c and 11c could induce the apoptosis of HeLa,SMMC-7721 and SGC-7901 cells.

Key words: 18β-glycyrrhetinic acid derivatives containing pyridine carboxamide; HeLa cell; SMMC-7721 cell; SGC-7901 cell

CLC Number:

R966

WU Yue, ZHANG Yu-yan, LI Xue-qiang, ZHOU Xue-zhang. Study on Anti-tumor Activity of 18β-glycyrrhetinic Acid Derivatives Containing Pyridine Carboxamide in vitro[J]. , 2015, 42(11): 2822-2832.

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Study on Anti-tumor Activity of 18β-glycyrrhetinic Acid Derivatives Containing Pyridine Carboxamide in vitro

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