迟钝爱德华菌PuAH基因的原核表达及其部分特性分析

doi:10.16431/j.cnki.1671-7236.2015.10.009

Abstract

Abstract: This assay was aimed to express the PuAH gene of Edwardsiella tarda (E.tarda) EIB202 and analyze its partial characteristics.A pair of specific primers was designed based on the PuAH gene sequence of E.tarda published in GenBank(accession No.:CP001135,CDS No.:ETAE_0818).The gene was cloned and expressed by recombinant plasmid pET32a-PuAH in E.coli BL21(DE3).The recombinant protein was purified and used to immune New Zealand rabbits to prepare antisera.Then the characteristics of expression product and antisera were analyzed by ELISA,Western blotting,hemolysis test and bacterial agglutination test.In conclusion,the expression product was fusion protein with 42 ku molecular weight,and had immunogenicity and antigenicity,but no hemolytic to erythrocytes of eel.Yet the antisera could inhibited the hemolytic of outer membrane protein of E.tarda EIB202 to some extent,and could agglutinate E.tarda EIB202.

Key words: E.tarda EIB202; PuAH gene; prokaryotic expression; antisera

CLC Number:

Q786

CHEN Bin, CHI Hong-shu, FANG Qin-mei, XU Bin-fu, GONG Hui. Prokaryotic Expression of Edwardsiella tarda PuAH Gene and Analysis of its Partial Characteristics[J]. , 2015, 42(10): 2573-2579.

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Prokaryotic Expression of Edwardsiella tarda PuAH Gene and Analysis of its Partial Characteristics

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