不同浓度神经肽P物质刺激对内蒙古白绒山羊皮肤干细胞BMP2、BMP4基因表达的影响

doi:10.16431/j.cnki.1671-7236.2015.09.012

Abstract

Abstract: This study was aimed to explore the expression of BMP2 and BMP4 genes in skin stem cells of Mongolia Cashmere goat with the influence of different concentrations of neuropeptide P (SP).We set five different SP concentrations (0, 1×10^-6, 1×10^-7, 1×10^-8 and 1×10^-9 mol/L) to stimulate the skin stem cells, then identified the expression quantities of BMP2 and BMP4 at days of 0, 7, 14 and 21 by Real-time quantitative PCR with the purpose of determining the optimum SP concentration for the differentiation of skin stem cells.The results showed that SP with the concentrations of 1×10^-6 and 1 ×10^-8 mol/L promoted the expression of BMP2 and BMP4;The expression of BMP2 at 7 and 14 d in SP 1×10^-8 mol/L group was significantly higher than that of the SP 1×10^-6 mol/L group (P<0.05);The expression of BMP4 in SP 1×10^-6 mol/L group had no significant difference with the SP 1×10^-8mol/L group (P>0.05);At 21 d, the expression of BMP2 and BMP4 significantly decreased in SP 1×10^-8 and the SP 1×10^-6mol/L groups, and the expression of BMP2 in the SP 1×10^-8 mol/L group was significantly higher than that of the 1×10^-6mol/L group (P<0.05), while the expression of BMP4 in the SP 1×10^-6 mol/L group had no significant difference with that of SP 1×10^-8 mol/L group (P>0.05).The results indicated that the concentrations of 1×10^-6 and 1×10^-8 mol/L not only might affect the expression of BMP2 and BMP4 of skin stem cells, but also provided a theoretical basis for the directional differentiation of skin stem cells.

Key words: skin stem cells; neuropeptide P; BMP; expression

CLC Number:

Q813.1⁺1

WANG Ya-juan, GAO Feng, LIU Ying-chun, ZHOU Huan-min. Effect of Different Concentrations of Neuropeptide P on Expression of BMP2 and BMP4 Genes in Skin Stem Cells of Mongolia Cashmere Goat[J]. , 2015, 42(9): 2298-2302.

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Effect of Different Concentrations of Neuropeptide P on Expression of BMP2 and BMP4 Genes in Skin Stem Cells of Mongolia Cashmere Goat

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