水貂阿留申病基因工程诊断抗原的制备

doi:10.16431/j.cnki.1671-7236.2015.07.010

Abstract

Abstract: To develop a new method of Aleutian disease (AD) diagnostic antigen production,we used Bac-to-Bac expression system in this study.Firstly,Aleutian disease virus (ADV) genome was extracted and ADV VP2 gene was amplified by PCR method.Then Bacmid-VP2 was constructed and transfected into insect cell Sf9 by liposomes to construct AcMVPV-VP2.Secondly,the VP2 protein was observed by electromicroscope and antigency was detected by Western blotting.At last,the activity of recombinant protein was inspected by countercurrent immunoelectrophoresis.The results showed that the expressed recombinant VP2 protein could react with ADV positive serum and form virus like particles.Compared with the commercial diagnostic antigen,the coincidence rate of recombinant antigen was 100%.This method could be a candidate for AD diagnostic antigen production.

Key words: mink Aleutian disease; genetic engineering; diagnostic antigen

CLC Number:

Q786

ZHANG Lei, CHAI Xiu-li, FENG Pei-ping, ZHANG Hai-ling, HU Bo, BAI Xue, ZHAO Jian-jun, FENG Zhuo, WANG Zhen-jun, XU Lei, YAN Xi-jun, WU Wei. Preparation of Genetic Engineering Diagnostic Antigen of Mink Aleutian Disease[J]. , 2015, 42(7): 1686-1691.

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Preparation of Genetic Engineering Diagnostic Antigen of Mink Aleutian Disease

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