蒙古绵羊Brachyury基因的克隆与生物信息学分析

doi:10.16431/j.cnki.1671-7236.2015.05.003

Abstract

Abstract: In order to study Mongolian sheep Brachyury gene DNA sequence, main purpose of this article by using the method of PCR, DNA sequence of Mongolian sheep Brachyury gene cloning and the complete Brachyury gene sequences were obtained for the first time.The sequencing with NCBI BLAST function on the site, comparing the results with the published sequences of sheep Brachyury homology was as high as 99%.We compared these sequences with ten Brachyury genes of human, mouse and other animals in GenBank database.The results showed that the most sequences were identical.Mongolian sheep Brachyury shared a 96.30% amino acid homology with Bos taurus, the highest degree of homology indicated a close genetic relationship, and the Caudata had the lowest homology 58.45%.It could be further confirmed by phylogenetic analysis.Moreover, the results showed that Mongolian sheep Brachyury gene sequence of amino acids and other vertebrates Brachyury gene sequence of amino acids were highly conservative.The research results showed that Mongolian sheep Brachyury gene sequences and other vertebrates Brachyury gene sequences were highly conservative.

Key words: Brachyury gene; vertebrates; cloning; sequence analysis

CLC Number:

Q785

REN Li-xin, ZHI Da-fu, LI Qi, CAO Gui-fang. Cloning and Sequence Analysis of Mongolian Sheep Brachyury Gene[J]. , 2015, 42(5): 1056-1062.

References

[1] 王生存, 缪进, 刘春, 等.短尾小鼠脊柱发育形态与病理生理学研究[J].交通医学, 2012, 26(6):527-532.
[2] 邵义祥, 陈兵, 张成香, 等.ENU 诱导获得一种短尾小鼠及其突变基因的初步定位[J].动物学杂志, 2005, 40(1):8-13.
[2] Bergh P, Kindblom L G, Gunterberg B, et al.Prognostic factors in chordoma of the sacrum and mobile spine:A study of 39 patients[J].Cancer, 2000, 88(9):2122-2134.
[4] Schwab J H, Boland P J, Agaram N P, et al.Chordoma and chondrosarcoma gene profile:Implications for immunotherapy[J]. Cancer Immunol Immunother, 2009, 58(3):339-349.
[5] Mcmaster M L, Goldstein A M, Bromley C M, et al.Chordoma:Incidence and survival patterns in the United States, 1973-1995 [J].Cancer Causes Control, 2001, 12(1):1-11.
[6] 矫健.T-Box转录因子Brachyury调控脊索瘤细胞凋亡的作用与分子机制[D].上海:第二军医大学, 2012.
[7] Oakley G J, Fuhrer K, Seethala R R.Brachyury, SOX-9, and podoplanin, new markers in the skull base chordoma vs chondrosarcoma differential:A tissue microarray-based comparative analysis[J].Modern Pathology, 2008, 21(12):1461-1469.
[8] Gruneberg H.Genetieal studies on the skeleton of the mouse.ⅩⅩⅢ.The development of Brachyury and Anury[J].Embryo Exp Morph, 1958, 132(6):424-443.
[9] Hoffmann A, Czichos S, Kaps C.The T-box transcription factor Brachyury mediates cartilage development in mesenchymal stem cell line C3H10T1/2[J].Cell Sci, 2002, 115(4):769-781.
[10] Knezevic V, Santo R D, Mackem S.Two novel chick T-box genes related to mouse Brachyury are expressed in different, non-overlapping mesodermal domains during gastrulation[J].Development, 1997, 12(24):411-419.
[11] Agulnik S I, Garvey N, Hancock S.Evolution of mouse T-box genes by tandem duplication and cluster dispersion[J].Genetics, 1996, 144(8):249-254.

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Cloning and Sequence Analysis of Mongolian Sheep Brachyury Gene

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