基因Ⅳ型戊型肝炎病毒开放阅读框3表达的蛋白(pORF3)对L02细胞基质金属蛋白酶(MMP)表达的影响

doi:10.16431/j.cnki.1671-7236.2015.04.005

Abstract

Abstract: To discuss the mechanism how open reading frame 3 (ORF3) protein of genotype Ⅳ hepatitis E virus (HEV) affected the expression of matrix metalloproteinase (MMP) in target cells, Human Matrix Metalloproteinase Antibody Array was used to compare and analyze the expression levels of matrix metalloproteinase in stable cell lines that expressed or did not express HEV ORF3, screening the matrix metalloproteinases which were down-regulated or up-regulated. The results indicated that the expressions of TIMP-1, TIMP-2 and TIMP-4 in stable L02 cell line with expression of pEGFP-ORF3 were down-regulated, and which provided important information for explaining the molecular mechanism through which HEV pORF3 affected the expression of protein associated with matrix metalloproteinase in target cells.

Key words: protein chip; hepatitis E; L02 cell; matrix metalloproteinase (MMP); expression

CLC Number:

Q786

GUO Shi-yu, SHI Qiao-yun, JIA Xiao-xiao, ZHU Hua-pei, ZHAO Tian-jing, PANG Feng, XU Kai-lian, LIANG Yuan-xiang, CUI Ke, DU Li, WU Ke-bang, WANG Feng-yang. Effects of Open Reading Frame 3 (ORF3) Expressed Protein of Genotype Ⅳ Hepatitis E Virus on the Expression of Matrix Metalloproteinase in L02 Cells[J]. , 2015, 42(4): 804-809.

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Effects of Open Reading Frame 3 (ORF3) Expressed Protein of Genotype Ⅳ Hepatitis E Virus on the Expression of Matrix Metalloproteinase in L02 Cells

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