用重组蛋白 NcSAG1t作为ELISA诊断抗原进行改良绒山羊Neospora caninum的血清学诊断

›› 2007, Vol. 34 ›› Issue (2): 109-111.

用重组蛋白 NcSAG1t作为ELISA诊断抗原进行改良绒山羊Neospora caninum的血清学诊断

陆艳,王戈平,马利青

青海省畜牧兽医科学院，西宁 810016

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-20 发布日期:2007-02-20

Serodiagnosis of Neospora Caninum Infection in Improvement Cashmere Goat by Enzyme-Linked Immunosorbent Assay with Recombinant Truncated NcSAG1t

LU Yan, WANG Geping, MA Liqing

Qinghai Academy of Animal and Veterinary Science, Xining 810016, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-20 Published:2007-02-20

摘要/Abstract

摘要： N caninum的表面抗原1（NcSAG1）在建立新孢子虫病的诊断上是一个重要的诊断抗原。为了建立有效的诊断方法，基因编码的截头（NcSAG1t）缺失一个信使缩氨酸和C-末端的疏水性区域的基因，象谷胱甘肽转移酶（GST）的融合蛋白一样被克隆和表达在escherichia coli。纯化的GST-NcSAG1t作为ELISA诊断抗原被用在改良绒山羊N caninum 抗体的检测中。经过对 207 份改良绒山羊血清样品进行检测，检出N caninum抗体阳性 16 份，阳性率为 7.73%。结果初步说明青海省的改良绒山羊羊群中存在N caninum。

关键词: The surface antigen 1 of N caninum (NcSAG1) is an important candidate for the development of a diagnostic reagent for neosporosis. In order to establish an effective diagnostic method; the gene encoding truncated NcSAG1 (NcSAG1t) lacking a signal peptide and C-terminal hydrophobic regions was cloned and expressed in Escherichia colias a fusion protein with glutathione Stransferase (GST). The purified GST-NcSAG1t was tested in an enzyme-linked immunosorbent assay (ELISA) for the detection of N caninumantibodies in Improvement Cashmere Goat. Field serum samples collected from Improvement Cashmere Goat in Delingha City of Qinghai province were examined for the diagnosis of neosporosis by using the ELISA. Of the 207 samples analyzed; 16 (7.73%) samples were positive for antibodies to N caninum. These results suggest that Neospora caninum is infected in Improvement Cashmere Goat from Qinghai area.

Abstract: N caninum；NcSAG1t；ELISA；改良绒山羊；抗体

Key words: neospora caninum; NcSAG1t; ELISA; improvement cashmere goat; serodiagnosis

中图分类号:

S852.71+3

陆艳;王戈平;马利青. 用重组蛋白 NcSAG1t作为ELISA诊断抗原进行改良绒山羊Neospora caninum的血清学诊断[J]. , 2007, 34(2): 109-111.

LU Yan;WANG Geping;MA Liqing. Serodiagnosis of Neospora Caninum Infection in Improvement Cashmere Goat by Enzyme-Linked Immunosorbent Assay with Recombinant Truncated NcSAG1t [J]. China Animal Husbandry & Veterinary Medicine, 2007, 34(2): 109-111.