猪抵抗素（resistin）ELISA检测方法的建立

›› 2007, Vol. 34 ›› Issue (6): 58-60.

猪抵抗素（resistin）ELISA检测方法的建立

冷扬1，2,刘铀2,刘艳芬3，王群3，潘颖斌3,高志杰3

1.广东医学院实验动物中心，湛江 524023；2.广东海洋大学现代生物化学中心，湛江 524088；3.广东海洋大学农学院，湛江 524088

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-06-20 发布日期:2007-06-20

The Establishment of ELISA Method for Detecting Porcine Resistin

LENG Yang1,2, LIU You2,LIU Yanfen3,WANG Qun3,PAN Yingbin3,GAO Zhijie3

1.Laboratery Animal Center of Guangdong Medical College,Zhanjiang 524023,China;2.Modern Biochemistry Center of Guangdong Ocean Univercity,Zhanjiang 524088,China;3.Agronomy College of Guangdong Ocean Univercity,Zhanjiang 524088,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-20 Published:2007-06-20

摘要/Abstract

摘要： 用纯化的可溶性重组resistin蛋白免疫健康家兔，制备抗血清，优化ELISA各反应条件（如工作浓度、温育时间、特异性试验），初步建立了检测猪resistin蛋白的间接ELISA方法。结果表明抗原、抗体最佳工作浓度为1∶200和1∶400，resistin最低检出限量为193.75 ng/ml；抗原和一抗、一抗和二抗的最适温育时间均为60 min。本试验建立的ELISA方法，具有灵敏度较高，特异性较好的特点，可作为猪组织和血清中resistin蛋白的检测方法。

关键词: 抵抗素; 重组蛋白：ELISA

Abstract: The purified resistin was used as antigen for immunizing rabbit to obtain resistin specific antisera. The ELISA for resistin detection is developed initially by optimizing conditions of ELISA action.The results showed that the working concentration of antigen-antibody was 1∶200 and 1∶400, the lowest detectability of resistin was about 193.75 ng/ml.The established ELISA method was shown to be sensitive,specific and reproducible.

Key words: resistin; recombinant protain; ELISA

中图分类号:

Q511

冷扬;刘铀;刘艳芬;王群;潘颖斌;高志杰 . 猪抵抗素（resistin）ELISA检测方法的建立 [J]. , 2007, 34(6): 58-60.

LENG Yang;LIU You;LIU Yanfen;WANG Qun;PAN Yingbin;GAO Zhijie . The Establishment of ELISA Method for Detecting Porcine Resistin [J]. , 2007, 34(6): 58-60.