1株H6N6亚型禽流感病毒贵州分离株HA和NA基因克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2016.02.002

›› 2016, Vol. 43 ›› Issue (2): 296-303.doi: 10.16431/j.cnki.1671-7236.2016.02.002

1株H6N6亚型禽流感病毒贵州分离株HA和NA基因克隆与序列分析

陈佳琪^1,2, 嵇辛勤^1,2, 段志强¹, 文明^1,2, 阮涌¹, 雷云^1,2, 侯萍³

1. 贵州大学动物科学学院, 贵阳 550025;
2. 贵州省动物疫病研究室, 贵阳 550025;
3. 贵州省种畜禽种质测定中心, 贵州贵阳 550025

收稿日期:2015-07-21 出版日期:2016-02-20 发布日期:2016-03-02
通讯作者: 嵇辛勤 E-mail:jxq972@aliyun.com
作者简介:陈佳琪(1988-),女,贵州长顺人,硕士生,研究方向:兽医公共卫生学,E-mail:jq85647616@126.com
基金资助:
贵州省规模养鸭场病原菌耐药性调查与控制研究(黔科合NY[2013]3074号);水禽免疫增强剂创制与开发关键技术研究(黔科合计省合[2013]7004号)

Cloning and Sequence Analysis of HA and NA Genes of One Strain of H6N6 Subtype Avian Influenza Virus Isolated from Guizhou Province

CHEN Jia-qi^1,2, JI Xin-qin^1,2, DUAN Zhi-qiang¹, WEN Ming^1,2, RUAN Yong¹, LEI Yun^1,2, HOU Ping³

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Laboratory for Animal Epidemic Disease of Guizhou, Guiyang 550025, China;
3.Kind of livestock and poultry species determination center of Guizhou, Guiyang 550025, China

Received:2015-07-21 Online:2016-02-20 Published:2016-03-02

摘要/Abstract

摘要： 为了解 H6亚型禽流感病毒(AIV)在贵州地区的流行情况,本研究对2014年从贵州省三穗鸭体内分离鉴定出的1株H6N6亚型AIV (A/duck/Guizhou/013/2014) HA和NA基因进行了克隆和序列分析。结果显示,A/duck/Guizhou/013/2014的HA基因与华东地区2009年鸭源H6N6亚型AIV同源性最高,达97.5%,HA蛋白裂解位点的氨基酸序列为P-Q-I-E-T-R-G,符合低致病性AIV的分子特征;而NA基因则与福建2007年鸭源H6N6亚型AIV同源性最高,达98.2%;由遗传进化树分析结果可知,HA和NA基因在遗传进化关系上,与湖南毒株位于同一分支,而与2007年贵州分离的3株H6N6亚型AIV不处于同一分支,说明A/duck/Guizhou/013/2014与本地区的H6N6亚型AIV亲缘关系较远。本研究结果表明当前贵州地区H6N6亚型AIV存在明显的遗传多样性。

关键词: 禽流感病毒; H6N6亚型; 血凝素基因; 神经氨酸酶基因; 序列分析

Abstract: To investigate the epidemic situation of H6N6 subtype avian influenza virus (AIV) in Guizhou province,A/duck/Guizhou/013/2014 was isolated from Sansui duck in live poultry market of Guizhou in 2014,the hemagglutinin (HA) and neuraminidase (NA) genes of DK/GZ/14 were subjected to clone and sequence analysis.The results showed that HA gene had the highest nucleotide homologies (97.5%) with the duck-origin H6N6 subtype AIV isolated from Eastern China in 2009,and the strains of HA gene proteolytic cleavage sites was P-Q-I-E-T-R-G,which accordeol with the molecular characteristic of low pathogenic AIV (LPAIV).However,NA gene of A/duck/Guizhou/013/2014 had the highest nucleotide homologies (98.2%) with the duck-origin H6N6 subtype AIV isolated from Fujian in 2007.The phylogenetic tree showed that A/duck/Guizhou/013/2014 and Hunan strains located in the same branch,while three duck-origin H6N6 subtype AIV isolated from Guizhou in 2007 and A/duck/Guizhou/013/2014 located in the different branch for HA and NA genes in genetic evolution,which suggested that A/duck/Guizhou/013/2014 was far with the local H6N6 subtype.The results also clearly indicated that duck-origin H6N6 subtype AIV had genetic diversity in duck population in Guizhou.

Key words: avian influenza virus; H6N6 subtype; HA gene; NA gene; sequence analysis

中图分类号:

陈佳琪, 嵇辛勤, 段志强, 文明, 阮涌, 雷云, 侯萍. 1株H6N6亚型禽流感病毒贵州分离株HA和NA基因克隆与序列分析[J]. , 2016, 43(2): 296-303.

CHEN Jia-qi, JI Xin-qin, DUAN Zhi-qiang, WEN Ming, RUAN Yong, LEI Yun, HOU Ping. Cloning and Sequence Analysis of HA and NA Genes of One Strain of H6N6 Subtype Avian Influenza Virus Isolated from Guizhou Province[J]. , 2016, 43(2): 296-303.

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1株H6N6亚型禽流感病毒贵州分离株HA和NA基因克隆与序列分析

Cloning and Sequence Analysis of HA and NA Genes of One Strain of H6N6 Subtype Avian Influenza Virus Isolated from Guizhou Province

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