羊痘病毒诊断学研究进展

中国畜牧兽医

羊痘病毒诊断学研究进展

王曼，卢昌，赵志荀，吴国华，颜新敏，朱海霞，李健，张强

中国农业科学院兰州兽医研究所，家畜疫病病原生物学国家重点实验室，国家口蹄疫参考实验室，甘肃省生物检测工程技术研究中心，甘肃兰州 730046

收稿日期:2014-01-13 出版日期:2014-07-20 发布日期:2014-08-21
通讯作者: 张强（1942—），男，陕西人，副研究员，从事兽医微生物及其分子生物学研究。E-mail：qzhang1616@sohu.com
作者简介:王曼（1989—），女，山东人，硕士生，研究方向：兽医微生物及其分子生物学。
基金资助:
甘肃省自然科学基金（1208RJZA101）；公益性行业科研专项（201310093-03）；国家“十二五”高技术研究发展计划（863）项目（2012AA101304）。

Research Progress on the Diagnostics of Capripoxvirus

WANG Man, LU Chang, ZHAO Zhi-xun, WU Guo-hua, YAN Xin-min, ZHU Hai-xia, LI Jian, ZHANG Qiang

Engineering Research Center of Biological Detection of Gansu Province, National Foot and Mouth Disease Reference Laboratory, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of CAAS, Lanzhou 730046, China

Received:2014-01-13 Online:2014-07-20 Published:2014-08-21

摘要/Abstract

摘要： 羊痘病毒是动物重要的痘病毒，属于痘病毒科脊椎动物痘病毒亚科，包括绵羊痘病毒、山羊痘病毒和疙瘩皮肤病毒。其引起的羊痘严重影响了养羊业和国际贸易的发展。作者介绍了羊痘病毒病原学特征、危害及临床症状，重点阐述了羊痘病毒分类检测的研究进展，并对新的检测方法应用于羊痘病毒分类检测进行了展望。

关键词:

羊痘病毒；分类；ELISA；RTmso-bidi-font-family: 宋体" lang="EN-US">-PCR

Abstract: Capripoxvirus, which belongs to Chordopoxvirinae genus, are important animal poxvirus. There are three members in capripoxvirus, sheep poxvirus, goat poxvirus and lumpy skin disease virus. And capripoxvirus seriously affect the development of wool-growingindustry and international trade. The pathogenic features and clinical symptoms of capripoxvirus were introduced in the present, as well as the research progress on the classification of capripoxvirus. Besides, the development of new detection technology was prospected.

Key words: capripoxvirus; classification; ELISA; RT-PCR

王曼，卢昌，赵志荀，吴国华，颜新敏，朱海霞，李健，张强. 羊痘病毒诊断学研究进展[J]. 中国畜牧兽医.

WANG Man, LU Chang, ZHAO Zhi-xun, WU Guo-hua, YAN Xin-min, ZHU Hai-xia, LI Jian, ZHANG Qiang. Research Progress on the Diagnostics of Capripoxvirus[J]. China Animal Husbandry & Veterinary Medicine.

参考文献

1 朱学亮，张强，杨帆，等.羊痘病毒载体研究进展［J］.中国畜牧兽医，2009，36（5）：98～101.
2 颜新敏，张强.羊痘病毒免疫学研究进展［J］.动物医学进展，2009a，30(12): 67～70.
3 颜新敏，张强，吴国华，等.两种用于鉴别山羊痘病毒和绵羊痘病毒方法的应用于比较研究［J］.华北农学报，2009b, 24(6)：50～53.
4 Babiuk S, Bowden T R, Parkyn G, et al. Yemen and Vietnam capripoxviruses demonstrate a distinct host preference for goats compared with sheep［J］. J Gen Virol,2009a,90(1):105～114.
5 Babiuk S, Wallace D B, Smith S J, et al. Detection of antibodies against capripoxviruses using an inactivated sheeppox virus ELISA［J］. Transbound Emerg Dis,2009b,56(4):132～141.
6 Bowden T R, Babiuk S L, Perkyn G R, et al. Capripoxvirsu tissue tropism and shedding: A quantitative study in experimentally infected sheep and goats［J］. Virology, 2008, 371(2):380～393.
7 Bowden T R, Coupar B E, Babiuk S L, et al. Detection of antibodies specific for sheeppox and goatpox viruses using recombinant capripoxvirus antigens in an indirect enzyme-linked immunosorbent assay［J］. J Virol Methods,2009,161(1):19～29.
8 Carm V M, Kitching R P, Hammond J M, et al. Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripoxvirus［J］. J Virol Methods,1994,49(3):285～294.
9 Chand P, Kitching R P, Black D N. Evaluation of the Western blot analysis of virus-specific antibody responses to capripox and contagious pustular dermatitis viral infection in sheep［J］.Epidemiol Infect,1994,113(2):337～385.
10 Das A, Babiuk S, Mclntosh M T. Development of a loop-mediated isothermal amplification assay for rapid detection of capripoxvirus［J］. J Clin Microbiol,2012,50(5):1613～1620.
11 Gelaye E, Larmien C E, Siber R, et al. Development of a cost-effective method for capripoxvirus genotyping using snapback primer and dsDNA inercalating dye［J］. PLoS One,2013,8(10):e75971.
12 Heine H G, Stevens M P, Foord A J, et al. A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homology of the vaccinia virus H3L gene［J］. J Immunol Methods,1999,227（1～2）:187～196.
13 Hollinshead M, Vanderplasschen A, Smith G L, et al. Vaccinia virus intracellular mature virions contain only one lipid membrane［J］. J Virol,1999,73(2):1503～1517.
14 Hosamani M, Mondal B, Tembhurne P A, et al. Differentiation of sheep pox and goat poxvirues by sequence analysis and PCR-RFLP of P32 gene［J］. Virus Genes,2004,29(1):73～80.
15 Lamien C E, Le Goff C, Siber R, et al. Use of the capripoxvirus homologue of vaccinia virus 30 kDa RNA polymerase subunit(RPO30) gene as a novel diagnostic and genotyping target: Development of a classical PCR method to differentiate goat poxvirus from sheep poxvirus［J］. Vet Microbiol,2011a,149(1～2):30～39.
16 Lamien C E, Lelenta M, Goger W, et al. Real time PCR method for simultaneous detection, quantitation and differentiation of capripoxviruses［J］. J Virol Methods,2011b,171(1):134～140.
17 Le Goff C, Lamien C E, Fakhfakh E, et al. Capripox G-protein-coupled chemokine receptor：A host-range gene suitable for virus-animal origin discrimination［J］. J Gen Virol,2009,90(8):1967～1977.
18 Montgomergry J L, Sanford L N, Wittwer C T. High-resolution DNA melting analysis in clinical research and diagnostics［J］. Expert Rev Mol Diagn,2010,10(2):219～240.
19 Moss B. Poxviridae: The virus and their replication［J］. Fields Virology,2007,2:2905～2946.
20 Moss B. Poxvirus DNA replication［J］. Cold Spring Harbor Perspectives in Biology,2013,5(9):a10119.
21 Murray L, Edwards L, Tuppurainen E S, et al. Detection of capripoxvirus DNA using a novel loop-mediated isothermal amplification assay［J］. BMC Vet Res,2013,1(9):90～95.
22 Smith G L, Vanderplasschen A, Law M. The formation and function of extracellular enveloped vaccinia virus［J］. J Gen Virol, 2002,83(12):2915～2931.
23 Smith G L, Murphy B J, Law M. Vaccinia virus motility［J］. Annu Rev Microbiol,2003,57:323～342.
24 Stam Y, Kuznetzoya L, Friedgut O, et al. The use of lumpy skin disease virus genome termini for detection and phylogennetic analysis［J］. J Virol Methods,2008,151(2):225～229.
25 Stanford M M, Mcfadden G, Karupiah G, et al. Immunopathogenesis of poxvirus infections: The impending storm［J］.Immunol Cell Biol,2007,85(2):93～102.
26 Tian H, Wu J, Chen Y, et al. Development of a SYBR green real-time PCR method for rapid detection of sheep pox virus［J］. Virol J,2012,27(9):291～294.
27 Tulmuan E R, Afonso C L, Lu Z, et al. The genomes of sheeppox and goatpox viruses［J］. J Virol,2002,76(12):6054～6061.
28 Venkatesan G, Balamurugan V, Yoqisharadhya R, et al. Differentiation of sheeppox and goatpox viruses by polymerase chain reaction-restriction fragment length polymorphism［J］. Virol Sin,2012,27(6):353～359.
29 Venkateasan G, Balamurugan V, Bhanuprakash V. Multiplex PCR for simultaneous detection and differentiation of sheeppox, goatpox and orf viruses from clinical samples of sheep and goats［J］. J Virol Methods,2014,195:1～8.
30 Zhou T, Jia H, Chen G, et al. Phylogenetic analysis of Chinese sheeppox and goatpox virus isolates［J］. Virol J,2012,20(9):25～33.
31 Zhu X L, Yang F, Li H X, et al. Identification and phylogenetic of a sheep pox virus isolated from the Ningxia Hui autonomous region of China［J］. Genet Mol Res,2013,14(2):1670～1678.