饲料中牛、羊源性成分现场可视化检测方法的建立

中国畜牧兽医

饲料中牛、羊源性成分现场可视化检测方法的建立

朱珠¹，兰青阔¹，赵新¹^，2，陈锐¹，郭永泽¹，王永¹^，3

1.天津市农业质量标准与检测技术研究所，天津 300381；2.天津大学化工学院，天津 300072；3.河北农业大学植物保护学院，河北保定 071001

收稿日期:2014-01-22 出版日期:2014-07-20 发布日期:2014-08-21
通讯作者: 王永。E-mail：wyzl2007@gmail.com
作者简介:朱珠(1963—），女，四川人，大专，助理研究员，研究方向：动物源性成分分子检测技术。
基金资助:
农业科技成果转化资金项目（2011GB2A100011）；科技部国际合作项目（2006DFA32380）。

Development of Visual Loop-mediate Isothermal Amplification Assay for Bovine and Ovine-derived Materials in Feedstuff

ZHU Zhu¹，LAN Qing-kuo¹，ZHAO Xin¹^，2，CHEN Rui¹，GUO Yong-ze¹，WANG Yong¹^，3

1.Tianjin Institute of Agricultural Quality Standard and Testing Technology，Tianjin 300381，China；2.School of Chemical Engineering，Tianjin University，Tianjin 300072，China；3.College of Plant Protection，Hebei Agricultural University，Baoding 071001，China

Received:2014-01-22 Online:2014-07-20 Published:2014-08-21

摘要/Abstract

摘要： 根据牛、羊线粒体中物种特异性序列设计引物，应用可视化环状等温扩增技术（visual loop-mediate isothermal amplification，vLAMP）对牛、羊源性成分进行快速扩增及可视化判断，同时建立牛、羊源成分人工污染的饲料模型，考察vLAMP检测方法的灵敏度。结果表明，牛源性引物和羊源性引物仅分别对牛、羊源性成分产生特异性扩增，灵敏度高达0.001%。本试验所建立的牛、羊源性成分现场可视化筛查方法具有较高的特异性与灵敏度，操作简单、快速，可用于饲料中牛、羊源性成分污染和食品掺伪的现场可视化检测。

关键词: 牛、羊源性成分; 环状等温扩增技术; 现场可视化检测

Abstract: Two set of primers were designed according to the mitochondrial DNA sequences of the targets，8 meat powder were detected by visual loop-mediate isothermal amplification （vLAMP） for method specificity，and meanwhile the mode of artificially contaminated feed was constructed to evaluate the sensitivity of vLAMP assay.The results showed that the bovine and ovine-derived materials had specific amplification，but the other animal origin submitted negative reactions.Sensitivity of vLAMP assay for bovine and ovine-derived materials was 0.001%.In conclusion，the vLAMP assay developed in the present study was a specific，sensitive，simple and convenient method for the rapid screening of bovine and ovine-derived materials in contaminated feeds.

Key words:

bovine and ovinemso-bidi-font-family: 宋体" lang="EN-US">-derived materials；loopmso-bidi-font-family: 宋体" lang="EN-US">-mediated isothermal amplification；visualization technology

朱珠，兰青阔，赵新，陈锐，郭永泽，王永. 饲料中牛、羊源性成分现场可视化检测方法的建立[J]. 中国畜牧兽医.

ZHU Zhu，LAN Qing-kuo，ZHAO Xin，CHEN Rui，GUO Yong-ze，WANG Yong. Development of Visual Loop-mediate Isothermal Amplification Assay for Bovine and Ovine-derived Materials in Feedstuff[J]. .

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