山羊组织蛋白酶L基因克隆、序列分析及组织表达研究

中国畜牧兽医

• 生物技术 • 下一篇

山羊组织蛋白酶L基因克隆、序列分析及组织表达研究

黄建文，王晓平，王念璐，徐刚毅

(四川农业大学，四川雅安 625000)

收稿日期:2014-01-16 出版日期:2014-07-20 发布日期:2014-08-21
通讯作者: 徐刚毅(1955—），男，四川人，研究员，博士生导师，研究方向：现代山羊养殖技术。E-mail：goatsxgy@263.net
作者简介:黄建文(1989—），男，四川人，硕士，研究方向：动物遗传育种与繁殖。
基金资助:
国家现代肉羊产业技术体系“十二五”项目（CARS-39）。

Cloning, Sequence Analysis and Tissue Expression of Goat CTSL Gene

HUANG Jian-wen，WANG Xiao-ping，WANG Nian-lu，XU Gang-yi

(Sichuan Agiricultural University,Ya’an 625000,China)

Received:2014-01-16 Online:2014-07-20 Published:2014-08-21

摘要/Abstract

摘要： 试验旨在克隆山羊组织蛋白酶L(cathepsin L，CTSL)基因,并进一步探讨该基因结构与功能的关系，揭示其组织表达规律。本研究以天府肉羊为试验材料，运用同源序列克隆技术，对山羊CTSL基因进行克隆，并对其进行生物信息学分析及组织表达研究。结果表明，山羊CTSL基因编码区(CDS区)长1005 bp，共编码334个氨基酸；山羊CTSL氨基酸与绵羊的同源性最高(99.10%),其次是牛(96.71%)、猪(90.12%)、人(76.95%)、大鼠(76.12%)、小鼠(75.82%)、青鳉鱼(66.17%)和斑马鱼(61.42%)；经预测山羊CTSL可能含有1个Inhibitor_129结构域和1个Pept_C1结构域；山羊CTSL基因在11个组织中均有表达，但在肺脏和肾脏中的表达量显著高于在心肌、肝脏、脾脏、胸肌、腹肌、腿肌、膈肌、眼肌和比目鱼肌中的表达量（P<0.05）。

关键词: font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">山羊；组织蛋白酶Lfont-size: 10.5pt; mso-bidi-font-family: 宋体; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">基因；克隆；序列分析；组织表达

Abstract: This study was enforced to acquire and analyze sequence of goat CTSL gene, and to uncover the expression of CTSL mRNA in different tissues of goat. On the basis of other species mRNA, the cDNA of CTSL gene in goat was amplified successfully by using RT-PCR. The structure and function of goat CTSL gene were analyzed by bioinformatics ways. The mRNA abundance of goat CTSL gene was detected in 11 tissues. The results revealed that goat CTSL gene cDNA code area was 1005 bp in length and encoded 334 amino acids. The amino acids homology of goat CTSL were 99.10%, 96.71%, 90.12%, 76.95%, 76.12%, 75.82%, 66.17% and 61.42% comparing to that of the ovis aries, cattle, pig, human, rattus, mouse, medaka and zebra fish, respectively. The CTSL amino acid sequence of goat included a Inhibitor_129 domain and a Pept_C1 domain. The mRNA expression level of goat CTSL gene was tested in all 11 tissues, but it was significantly higher in lung and in kidney than in heart, liver, spleen, pectoral muscle, abdominal muscle, crureus, diaphragm, ocular muscle and soleus（P<0.05）.

Key words: font-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA" lang="EN-US">goatfont-size: 10.5pt; mso-bidi-font-family: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">；CTSL gene；cloning；sequence analysis；tissue expression

黄建文，王晓平，王念璐，徐刚毅. 山羊组织蛋白酶L基因克隆、序列分析及组织表达研究[J]. 中国畜牧兽医.

HUANG Jian-wen，WANG Xiao-ping，WANG Nian-lu，XU Gang-yi. Cloning, Sequence Analysis and Tissue Expression of Goat CTSL Gene[J]. China Animal Husbandry & Veterinary Medicine.

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山羊组织蛋白酶L基因克隆、序列分析及组织表达研究

Cloning, Sequence Analysis and Tissue Expression of Goat CTSL Gene

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