中国畜牧兽医

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牛乳中α-酪蛋白完全抗原的合成及多克隆抗体的制备

褚晓红,周玉,李岩松,孟星宇,张莹,卢士英,任洪林,胡盼,柳增善,王男男,李丹   

  1. (人兽共患病研究教育部重点实验室,吉林大学人兽共患病研究所,吉林大学动物医学学院,吉林长春 130062)
  • 修回日期:2013-11-22 出版日期:2014-02-20 发布日期:2014-03-27
  • 通讯作者: 周玉。E-mail:zhouyu69@sina.com
  • 作者简介:褚晓红(1986-),女,山东人,硕士生,研究方向:兽医公共卫生。
  • 基金资助:

    国家自然科学基金(61171022、60971011)。

Synthesis of Complete Antigen and Preparation of Polyclonal Antibody against α-casein in Bovine Milk

CHU Xiao-hong, ZHOU Yu, LI Yan-song, MENG Xing-yu, ZHANG Ying, LU Shi-ying, REN Hong-lin, HU Pan, LIU Zeng-shan, WANG Nan-nan, LI Dan   

  1. Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University,Changchun 130062, China)
  • Revised:2013-11-22 Online:2014-02-20 Published:2014-03-27

摘要: 为研制α-酪蛋白多克隆抗体,以商品化α-酪蛋白为基础,通过戊二醛法制备完全抗原:免疫原(α-酪蛋白-BSA)和检测原(α-酪蛋白-OVA)。通过变性与非变性PAGE电泳对完全抗原进行鉴定。免疫新西兰大白兔,获得针对α-酪蛋白的多克隆抗体,经初步鉴定抗体效价为1∶256000,与κ-酪蛋白存在明显的交叉反应。本研究制备的多克隆抗体可用于检测牛乳中α-酪蛋白和κ-酪蛋白的总量。

关键词: α -酪蛋白; 完全抗原; 多克隆抗体

Abstract: In order to gain polyclonal antibody against α-casein, the commercial α-casein were linked with carrier protein BSA and OVA, respectively, as immunogen (α-casein-BSA) and coating antigen (α-casein-OVA). The reaction products were identified by nondenaturing PAGE and SDS-PAGE. New Zealand White rabbits were immunized with the immunogen (α-casein- BSA) to produce polyclonal antibody. The results showed that the titer of the antibody was 1∶256000 and had high cross reactivity with κ-casein. It could be used to detect the total amount of α-casein and κ-casein in milk.

Key words: α-casein; complete antigen; polyclonal antibody