胰岛素样生长因子结合蛋白3和胰岛素样生长因子结合蛋白5基因在内蒙古白绒山羊皮肤中的表达研究

›› 2013, Vol. 40 ›› Issue (10): 1-5.

• 生物技术 • 下一篇

胰岛素样生长因子结合蛋白3和胰岛素样生长因子结合蛋白5基因在内蒙古白绒山羊皮肤中的表达研究

于新蕾¹, 蔡婷¹, 俎红丽¹, 杨惠茹¹, 肖红梅¹, 刘志红¹, 王全喜², 王志新¹, 李金泉¹

1. 内蒙古农业大学动物科学学院, 动物遗传育种与繁殖重点实验室, 内蒙古呼和浩特 010018;
2. 内蒙古农业大学研究生院, 内蒙古呼和浩特 010018

收稿日期:2013-02-26 出版日期:2013-10-20 发布日期:2013-12-19
通讯作者: 李金泉 E-mail:lijinquan_nd@126.com
作者简介:于新蕾(1987-),女,内蒙古人,硕士生,研究方向:动物遗传繁殖与育种学。
基金资助:
国家863计划(2013AA102506);国家自然科学基金(31072005,31272421);国家农业部现代农业产业技术体系(CARS-40-05);内蒙古自治区自然科学基金(2013MS0411);教育部博士点专项基金(2012151520009)。

Study on Expression of Insulin-like Growth Factor-binding Protein -3 and -5 (IGFBP-3 and IGFBP-5) in the Skin of Inner Mongolia Cashmere Goat

YU Xin-lei¹, CAI Ting¹, JU Hong-li¹, YANG Hui-ru¹, XIAO Hong-mei¹, LIU Zhi-hong¹, WANG Quan-xi², WANG Zhi-xin¹, LI Jin-quan¹

1. Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Graduate School, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2013-02-26 Online:2013-10-20 Published:2013-12-19

摘要/Abstract

摘要： 本研究旨在探明胰岛素样生长因子结合蛋白(insulin-like growth factor-binding protein,IGFBP)在内蒙古白绒山羊绒毛发育的不同生长阶段皮肤组织中的表达变化情况。试验采用实时荧光定量PCR技术,对内蒙古白绒山羊绒毛生长发育的各个时期皮肤中IGFBP-1~IGFBP-6基因的表达进行了测定,并通过埋植褪黑素组改变绒毛生长周期的研究,来鉴定IGFBP-1~IGFBP-6基因中高表达的结合蛋白的作用。结果表明,IGFBP-3和IGFBP-5在绒山羊绒毛生长发育的各个时期的皮肤组织中均有表达,但是IGFBP-5的表达相对于IGFBP-3的表达具有很强的规律性。结果提示,在内蒙古白绒山羊的绒毛发育过程中,IGFBP-5可能是一个主要的调控因子。

关键词: 内蒙古白绒山羊; IGFBP-3; IGFBP-5; 毛囊周期

Abstract: This experiment was conducted to study the expression effect of insulin-like growth factor-binding protein(IGFBP) of Inner Mongolia Cashmere goat skin in different developmental stage. The expression of IGFBP-1 to IGFBP-6 in different developmental stage of hair follicle cycle was measured by Real-time PCR. Furthermore, the hair growth cycle was changed by implanting melatonin to identify the role of IGFBP-1 to IGFBP-6 gene, the high expression of the binding protein. The results showed that IGFBP-3 and IGFBP-5 both were expressed in different developmental stage of hair follicle cycle, however, the expression of IGFBP-5 gene presented more regular. The results indicated that IGFBP-5 might be a major regulatory factor in the process of hair follicle development of Inner Mongolia Cashmere goat.

Key words: Inner Mongolia Cashmere goat; IGFBP-3; IGFBP-5; hair follicle cycle

中图分类号:

于新蕾, 蔡婷, 俎红丽, 杨惠茹, 肖红梅, 刘志红, 王全喜, 王志新, 李金泉. 胰岛素样生长因子结合蛋白3和胰岛素样生长因子结合蛋白5基因在内蒙古白绒山羊皮肤中的表达研究[J]. , 2013, 40(10): 1-5.

YU Xin-lei, CAI Ting, JU Hong-li, YANG Hui-ru, XIAO Hong-mei, LIU Zhi-hong, WANG Quan-xi, WANG Zhi-xin, LI Jin-quan. Study on Expression of Insulin-like Growth Factor-binding Protein -3 and -5 (IGFBP-3 and IGFBP-5) in the Skin of Inner Mongolia Cashmere Goat[J]. , 2013, 40(10): 1-5.

参考文献

1 王林枫, 卢德勋, 孙海洲, 等. 光照和褪黑激素对内蒙古绒山羊氮分配和产绒性能的影响[J]. 中国农业科学, 2006(5):1004~1010.
2 李金泉, 尹俊, 周欢敏. 绒山羊绒毛生长调控机理研究进展[J]. 中国草食动物, 2001(3):41~44.
3 郑竹清,石玲,王梦一,等. 褪黑激素受体1a基因在内蒙古绒山羊垂体中的表达[J]. 中国畜牧兽医, 2012, 39(4): 39~41.
4 柳建昌, 尹协镇, 方天祺. 褪黑素对中国绒山羊在非生绒期促绒生长与绒产量的影响[J]. 动物学杂志, 1998(3):9~13.
5 Batch J A, Mercuri F A, Werther G A. Identification and localization of insulin-like growth factor-binding protein (IGFBP) messenger RNAs in human hair follicle dermal papilla[J]. J Invest Dermatol, 1996,106(3):471~475.
6 Boisclair Y R, Rhoads R P, Ueki I, et al. The acid-labile subunit (ALS) of the 150 kDa IGF-binding protein complex: An important but forgotten component of the circulating IGF system[J]. J Endocrinol, 2001,170(1):63~70.
7 Braheem M, Galbraith H, Scaife J, et al. Growth of secondary hair follicles of the Cashmere goat in vitro and their response to prolactin and melatonin[J]. J Anat, 1994,185 (Pt1):135~142.
8 Denley A, Cosgrove L J, Booker G W, et al. Molecular interactions of the IGF system[J]. Cytokine Growth Factor Rev, 2005,16(4~5):421~439.
9 Firth S M, Baxter R C. Cellular actions of the insulin-like growth factor binding proteins[J]. Endocr Rev, 2002a,23(6):824~854.
10 Firth S M, McDougall F, McLachlan A J, et al. Impaired blockade of insulin-like growth factor Ⅰ (IGF-Ⅰ)-induced hypoglycemia by IGF binding protein-3 analog with reduced ternary complex-forming ability[J]. Endocrinology, 2002b,143(5):1669~1676.
11 Kamalati T, Howard M, Brooks R F. IGF-Ⅰ induces differentiation in a transformed human keratinocyte line[J]. Development, 1989,106(2):283~293.
12 Little J C, Redwood K L, Granger S P, et al. In vivo cytokine and receptor gene expression during the rat hair growth cycle. Analysis by semi-quantitative RT-PCR[J]. Exp Dermatol, 1996,5(4):202~212.
13 Nixon A J, Choy V J, Parry A L, et al. Fiber growth initiation in hair follicles of goats treated with melatonin[J]. J Exp Zool, 1993a,267(1):47~56.
14 Nixon A J. A method for determining the activity state of hair follicles[J]. Biotech Histochem, 1993b,68(6):316~325.
15 Philpott M P, Sanders D A, Kealey T. Effects of insulin and insulin-like growth factors on cultured human hair follicles: IGF-Ⅰ at physiologic concentrations is an important regulator of hair follicle growth in vitro[J]. J Invest Dermatol, 1994,102(6):857~861.
16 Salih D A, Tripathi G, Holding C, et al. Insulin-like growth factor-binding protein 5 (IGFBP5) compromises survival, growth, muscle development, and fertility in mice[J]. Proc Natl Acad Sci USA, 2004,101(12):4314~4319.
17 Schlake T, Beibel M, Weger N, et al. Major shifts in genomic activity accompany progression through different stages of the hair cycle[J]. Gene Expr Patterns, 2004,4(2):141~152.
18 Tavakkol A, Elder J T, Griffiths C E, et al. Expression of growth hormone receptor, insulin-like growth factor Ⅰ (IGF-Ⅰ) and IGF-Ⅰ receptor mRNA and proteins in human skin[J]. J Invest Dermatol, 1992,99(3):343~349.
19 Twigg S M, Kiefer M C, Zapf J, et al. Insulin-like growth factor-binding protein 5 complexes with the acid-labile subunit. Role of the carboxyl-terminal domain[J]. J Biol Chem, 1998,273(44):28791~28798.
20 Weger N, Schlake T. IGFBP3 modulates cell proliferation in the hair follicle[J]. J Invest Dermatol, 2005,125(4):847~849.

[1]	吐尔逊江·吾木尔艾力, 孟军, 王建文, 曾亚琦, 姚新奎, 李林玲, 姚芳芳, 王欢, 任海帆, 黄晶晶, 阿米娜. 哈萨克马和焉耆马不同组织中IGFBP-5基因表达差异研究[J]. 《中国畜牧兽医》, 2016, 43(9): 2257-2264.
[2]	王彩霞, 刘少卿, 李金泉, 代盛超, 王志英, 张燕军, 王瑞军. 母羊胎次与配种日期对内蒙古白绒山羊产羔数的影响[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(6): 1512-1517.
[3]	王彩霞, 李金泉, 代盛超, 王志英, 周娟娟, 魏永龙, 孟瑞强, 张文广, 张燕军, 苏蕊, 刘志红, 王瑞军. 内蒙古白绒山羊产羔数遗传评估的研究[J]. , 2015, 42(1): 179-184.
[4]	周娟娟, 唐培荣, 王志新, 魏永龙, 孟瑞强, 王志英, 张文广, 张燕军, 苏蕊, 刘志红, 王瑞军, 李金泉. 内蒙古白绒山羊抓绒性状变化规律的研究[J]. , 2013, 40(9): 169-175.
[5]	李青, 刘学, 刘敏, 张跟喜, 丁馥香, 张丽, 王金玉. 边鸡胰岛素生长因子结合蛋白-3基因外显子2MspⅠ位点对繁殖性状的遗传效应分析[J]. , 2013, 40(2): 130-132.
[6]	柴局;张燕军;张家新;高日明;孟克格日乐;王惠梅;张文广;李金泉. 内蒙古白绒山羊附睾精子冷冻效果的研究[J]. , 2011, 38(10): 99-105.
[7]	金崇富;王金玉;王慧华;张跟喜;施会强;俞亚波. 京海黄鸡IGFBP-3基因外显子1和内含子1部分序列多态性及其与生长繁殖性状的相关性[J]. , 2010, 37(9): 132-134.

胰岛素样生长因子结合蛋白3和胰岛素样生长因子结合蛋白5基因在内蒙古白绒山羊皮肤中的表达研究

Study on Expression of Insulin-like Growth Factor-binding Protein -3 and -5 (IGFBP-3 and IGFBP-5) in the Skin of Inner Mongolia Cashmere Goat

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价