芦苇秸秆氧化钙和碳酸钠预处理工艺优化及结构分析

doi:10.16431/j.cnki.1671-7236.2023.09.012

中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (9): 3562-3572.doi: 10.16431/j.cnki.1671-7236.2023.09.012

芦苇秸秆氧化钙和碳酸钠预处理工艺优化及结构分析

孙竹文^1,2,3,4, 刘正群^2,3,4, 朱龙博^1,2,3,4, 李宁^2,3,4, 刘燕蓉^1,2,3,4, 梁世岳^2,3,4, 郑梓^2,3,4, 穆淑琴^2,3,4, 闫峻^2,3,4, 孙超¹

1. 西北农林科技大学动物科技学院, 杨凌 712100;
2. 天津市农业科学院畜牧兽医研究所, 天津 300381;
3. 天津市畜禽分子育种与生物技术重点实验室, 天津 300381;
4. 天津市畜禽健康养殖工程技术中心, 天津 300381

收稿日期:2023-02-06 出版日期:2023-09-05 发布日期:2023-08-24
通讯作者: 闫峻, 孙超 E-mail:yjjsxz@163.com;sunchao2775@163.com
作者简介:孙竹文,E-mail:2223594946@qq.com。
基金资助:
天津市中央引导地方科技发展专项(22ZYCGSN00130);天津市科技重大专项与工程项目(19ZXZYSN00120);天津市生猪产业技术体系创新团队母仔猪营养岗(ITTPRS2021005);甘肃省民生科技专项—乡村振兴专题项目(2021-0306-NCC-0180)

Process Optimization and Structure Analysis of Calcium Oxide and Sodium Carbonate Pretreatment of Reed Straw

SUN Zhuwen^1,2,3,4, LIU Zhengqun^2,3,4, ZHU Longbo^1,2,3,4, LI Ning^2,3,4, LIU Yanrong^1,2,3,4, LIANG Shiyue^2,3,4, ZHENG Zi^2,3,4, MU Shuqin^2,3,4, YAN Jun^2,3,4, SUN Chao¹

1. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China;
2. Institute of Animal Science and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China;
3. Tianjin Key Laboratory of Animal Molecular Breeding and Biotechnology, Tianjin 300381, China;
4. Tianjin Engineering Research Center of Animal Healthy Farming, Tianjin 300381, China

Received:2023-02-06 Online:2023-09-05 Published:2023-08-24

摘要/Abstract

摘要： 【目的】探究氧化钙(CaO)和碳酸钠(Na₂CO₃)联合预处理枯叶期芦苇秸秆的木质素降解最佳工艺条件及其结构变化。【方法】试验在添加2% Na₂CO₃的基础上,选取处理时间、处理温度、料液比和CaO添加量4个因素进行单因素梯度试验,探究其对木质素及半纤维素去除率的影响;根据单因素试验结果进行Box-Behnken响应面优化试验,探究木质素降解的最佳预处理条件;随后通过扫描电镜(SEM)、傅里叶红外光谱(FTIR)和X-射线衍射(XRD)分析芦苇秸秆在最佳预处理条件下的结构变化。【结果】不同处理时间、处理温度和CaO添加量对芦苇秸秆木质素和半纤维素去除率均有显著影响(P＜0.05),不同料液比无显著影响(P＞0.05);响应面优化结果显示回归模型极显著(P＜0.01),3种因素对木质素去除率均有极显著影响(P＜0.01);3种因素对木质素去除率影响由大到小依次为处理温度、CaO添加量、处理时间。Na₂CO₃添加量为2%、料液比1∶3时,预处理最佳工艺条件为:CaO添加量8.44%,处理时间5.52 h,处理温度49.58 ℃,在此工艺条件下芦苇秸秆木质素去除率的模型预测值为24.97%,试验验证结果为23.75%,与预测值接近。优化条件处理后,SEM结果显示芦苇秸秆表面发生破损,孔隙增大、增多;FTIR结果显示,芦苇秸秆的半纤维素、木质素对应化学键的吸收峰强度均降低;XRD结果显示,芦苇秸秆的纤维素相对结晶指数(CrI)由37.21%变为46.66%。【结论】采用响应面法优化CaO及Na₂CO₃联合预处理芦苇秸秆的工艺条件合理可行,预处理后的芦苇秸秆其表面形态结构、木质纤维素的化学结构和纤维素结晶结构均发生了明显变化。本试验结果可为非常规饲料资源高值化利用提供技术支撑。

关键词: 芦苇秸秆; 预处理; 响应面优化; 木质素去除率; 结构分析

Abstract: 【Objective】 The experiment aimed to investigate the optimal process conditions for lignin degradation of reed straw at the dead leaf stage by combined pretreatment with calcium oxide (CaO) and sodium carbonate (Na₂CO₃) and its structural changes.【Method】 The experiment was conducted to investigate the removal of lignin and hemicellulose by selecting four factors:Treatment time,treatment temperature,feed-to-liquid ratio,and CaO addition on the basis of 2% Na₂CO₃ addition in a gradient test.The Box-Behnken response surface optimization test was conducted to investigate the optimal pretreatment process for lignin degradation based on the results. Subsequently,the structural changes of reed under the optimal treatment conditions were analyzed by scanning electron microscopy (SEM),Fourier infrared spectroscopy (FTIR),and X-ray diffraction (XRD).【Result】 The lignin and hemicellulose removal rates of reed straw differed significantly (P＜0.05) at different treatment times,treatment temperatures and CaO additions,and there were no significant differences at different feed-to-liquid ratios (P＞0.05).The response results showed that the regression model was extremely significant (P＜0.01),and all three factors had extremely significant effects on lignin removal rate (P＜0.01),and the magnitude of the effects of the three factors on lignin removal rate were treatment temperature,CaO addition,and treatment time in order.The optimal pretreatment process conditions were 8.44% CaO addition,5.52 h treatment time,and 49.58 ℃ treatment temperature when Na₂CO₃ addition was 2% and material-to-liquid ratio was 1∶3.Under this pretreatment condition,the lignin removal rate of reed straw reached 23.75%,which was close to the model predicted value (24.97%). After optimization, SEM results showed that the surface of reed straw was broken and the pores were enlarged and increased;FTIR results showed that the absorption peak intensities of hemicellulose and lignin corresponding to chemical bonds of reed straw were reduced;XRD results showed that the relative crystallinity index (CrI) of cellulose of reed straw changed from 37.21% to 46.66%.【Conclusion】 The process conditions of combined pretreatment of reed straw with CaO and Na₂CO₃ by response surface methodology were reasonable and feasible.The surface morphological structure,chemical structure of lignocellulose and cellulose crystalline structure of the pretreated reed straw were significantly changed. The results could provide technical support for the high-value development and utilization of non-conventional feed resources.

Key words: reed straw; pretreatment; response surface optimization; lignin removal rate; structure analysis

中图分类号:

S816.5⁺1

孙竹文, 刘正群, 朱龙博, 李宁, 刘燕蓉, 梁世岳, 郑梓, 穆淑琴, 闫峻, 孙超. 芦苇秸秆氧化钙和碳酸钠预处理工艺优化及结构分析[J]. 中国畜牧兽医, 2023, 50(9): 3562-3572.

SUN Zhuwen, LIU Zhengqun, ZHU Longbo, LI Ning, LIU Yanrong, LIANG Shiyue, ZHENG Zi, MU Shuqin, YAN Jun, SUN Chao. Process Optimization and Structure Analysis of Calcium Oxide and Sodium Carbonate Pretreatment of Reed Straw[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3562-3572.

参考文献

[1] 张婷,周睿,陈雨生.我国饲料粮供需缺口估算研究[J].山东农业科学,2022,54(8):158-165. ZHANG T,ZHOU R,CHEN Y S.Estimation of supply-demand gap of feed grain in China[J].Shandong Agricultural Sciences,2022,54(8):158-165.(in Chinese)
[2] 赵金标,周行健,李佳,等.非常规饲料原料营养价值及其在生猪高效养殖中的应用策略[J].中国猪业,2022,17(2):17-23. ZHAO J B,ZHOU X J,LI J,et al.Efficient applications of unconventional feed ingredients in swine production[J].China Swine Industry,2022,17(2):17-23.(in Chinese)
[3] 中华人民共和国海关总署.2022年12月全国进口重点商品量值表[EB/OL].(2023-01-13)[2023-03-06].http://www.customs.gov.cn//customs/302249/zfxxgk/2799825/302274/302275/4794311/index.html. GENERAL ADMINISTRATION OF CUSTOMS OF THE PEOPLE’S REPUBLIC OF CHINA.The quantity table of key national import commodities in December 2022[EB/OL].(2023-01-13)[2023-03-06].http://www.customs.gov.cn//customs/302249/zfxxgk/2799825/302274/302275/4794311/index.html.(in Chinese)
[4] 郭春秀,李发明,张莹花,等.河西走廊芦苇草地资源特征及其保护利用[J].草原与草坪,2012,32(4):93-96. GUO C X,LI F M,ZHANG Y H,et al.Conservation and utilization of reed grassland resources in Hexi Corridor[J].Grassland and Turf,2012,32(4):93-96.(in Chinese)
[5] 张茜,裘天航,王安安,等.北京地区芦苇资源状态及其多样性[J].植物学报,2020,55(6):693-704. ZHANG Q,QIU T H,WANG A A,et al.Morphology and genetic diversity of phragmites australis in Beijing[J].Chinese Bulletin of Botany,2020,55(6):693-704.(in Chinese)
[6] 康翠翠,田川尧,王晓敏,等.芦苇的营养特性及可青贮性研究[J].延边大学农学学报,2016,38(2):149-155. KANG C C,TIAN C Y,WANG X M,et al.Nutritive peculiarity and silage study of phragmites australis[J].Agricultural Science Journal of Yanbian University,2016,38(2):149-155.(in Chinese)
[7] 高凤芹,陶雅,吴洪新,等.稀碱预处理对杂交狼尾草木质纤维素降解的影响[J].中国草地学报,2020,42(1):58-67. GAO F Q,TAO Y,WU H X,et al.Effect of dilute alkali pretreatment on lignocellulose degradation of hybrid pennisetum[J].Chinese Journal of Grassland,2020,42(1):58-67.(in Chinese)
[8] 陈鑫东,熊莲,黎海龙,等.低共熔溶剂在木质纤维素预处理促进酶水解效率的研究进展[J].新能源进展,2019,79(5):416-423. CHEN X D,XIONG L,LI H L,et al.Research progress of deep eutectic solvent in lignocellulose pretreatment to promote enzymatic hydrolysis efficiency[J].Advances in New and Renewable Energy,2019,79(5):416-423.(in Chinese)
[9] XU J,CHENG J J.Pretreatment of switchgrass for sugar production with the combination of sodium hydroxide and lime[J].Bioresource Technology,2011,102(4):3861-3868.
[10] SHI H T,CAO Z J,WANG Y J,et al.Effects of calcium oxide treatment at varying moisture concentrations on the chemical composition,in situ degradability,in vitro digestibility and gas production kinetics of anaerobically stored corn stover[J].Journal of Animal Physiology & Animal Nutrition,2016,100(4):748-757.
[11] COOK D E,BENDER R W,SHINNERS K J,et al.The effects of calcium hydroxide-treated whole-plant and fractionated corn silage on intake,digestion,and lactation performance in dairy cows[J]. Journal of Dairy Science,2016,99(7):5385-5393.
[12] 杨晓瑞,孙姚瑶,马艺鸣,等.稻草秸秆的预处理方法研究进展[J].粮食与油脂,2022,35(8):16-19. YANG X R,SUN Y Y,MA Y M,et al.Research progress on pretreatment of rice straw[J].Cereals & Oils,2022,35(8):16-19.(in Chinese)
[13] CHEN Y,STEVENS M A,ZHU Y,et al.Understanding of alkaline pretreatment parameters for corn stover enzymatic saccharification[J].Biotechnology for Biofuels,2013,6(1):8.
[14] SEGAL L,CREELY J J,MARTIN A E,et al.An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer[J].Textile Research Journal,1959,29(10):786-794.
[15] HE Y F,PANG Y Z,LIU Y P,et al.Physicochemical characterization of rice straw pretreated with sodium hydroxide in the solid state for enhancing biogas production[J].Energy & Fuels,2008,22(4):2775-2781.
[16] 寇巍,赵勇,闫昌国,等.膨化预处理玉米秸秆提高还原糖酶解产率的效果[J].农业工程学报,2010,26(11):265-269. KOU W,ZHAO Y,YAN C G,et al.Corn straw expansion pretreatment to improve enzymolysis reducing sugar yield[J].Transactions of the Chinese Society of Agricultural Engineering,2010,26(11):265-269.(in Chinese)
[17] 王平.玉米秸秆中木质纤维素的高效降解及其生物学效价评定的研究[D].郑州:河南农业大学,2016. WANG P. Study on high-efficient degradation of lignocellulose in corn straw and its biological value evaluation[D].Zhengzhou:Henan Agricultural University,2016. (in Chinese)
[18] ZHOU X, LI Q, ZHANG Y, et al. Effect of hydrothermal pretreatment on Miscanthus anaerobic digestion[J]. Bioresource Technology, 2017,224:721-726.
[19] 岳建芝.超微化秸秆粉体物性微观结构及光合生物产氢实验研究[D].郑州:河南农业大学,2011. YUE J Z.Properties,microstructure of ultrafine straw powder and hydrogen production from it by photosynthetic bacteria[D].Zhengzhou:Henan Agricultural University,2011.(in Chinese)
[20] 吴刚刚. 核壳结构Al@PDA@MOFs复合材料的制备及性能研究[D].南京:南京理工大学,2020. WU G G. The preparation and performance of core-shell Al@PDA@MOFs composites[D]. Nanjing: Nanjing University of Science & Technology,2019. (in Chinese)
[21] SAINI J K,PATEL A K,ADSUL M,et al.Cellulase adsorption on lignin:A roadblock for economic hydrolysis of biomass[J].Renewable Energy,2016,98:29-42.
[22] QIN C R,CLARKE K,LI K C.Interactive forces between lignin and cellulase as determined by atomic force microscopy[J].Biotechnology for Biofuels,2014,7:65.
[23] 吕文龙.益生菌与石灰处理秸秆对绵羊瘤胃发酵及消化性能的影响[D].哈尔滨:东北农业大学,2011. LYU W L.Effect of combination treatments of corn straw with probiotics and lime on rumen fermentation and diet nutrient digestion in sheep[D].Harbin:Northeast Agricultural University,2011.(in Chinese)
[24] 杨洁,杨雪琦,韩鲁佳.不同NaOH/球磨复合预处理对玉米秸秆酶解效果的影响[J].农业工程学报,2022,38(15):226-233. YANG J,YANG X Q,HAN L J.Effects of different NaOH/ball milling combined pretreatments on the enzymatic hydrolysis of corn stalks[J].Transactions of the Chinese Society of Agricultural Engineering,2022,38(15):226-233.(in Chinese)
[25] YANG Z,ZHANG M M,XIN D L,et al.Evaluation of aqueous ammonia pretreatment for enzymatic hydrolysis of different fractions of bamboo shoot and mature bamboo[J].Bioresource Technology,2014,173:198-206.
[26] SUN Z P,GE X Y,XIN D L,et al.Hydrolyzabilities of different corn stover fractions after aqueous ammonia pretreatment[J].Applied Biochemistry and Biotechnology,2014,172:1506-1516.
[27] 麻晓霞,蔡彦,马玉龙.碱/高温湿热法预处理玉米秸秆纤维素的研究[J].石油化工应用,2020,39(8):104-109. MA X X,CAI Y,MA Y L.Study on pretreatment of corn straw cellulose with alkali/steam[J].Petrochemical Industry Application,2020,39(8):104-109.(in Chinese)
[28] 钱文静,黄引超,王先柳,等.汽爆加工与碱化贮存提高沙棘枝干的饲用价值[J].饲料工业,2023,44(1):18-24. QIAN W J,HUANG Y C,WANG X L,et al.Steam explosion processing and alkalization storage improve the feeding value of Hippophae rhamnoides[J].Feed Industry,2023,44(1):18-24.(in Chinese)
[29] ZHENG M,LI X,LI L,et al.Enhancing anaerobic biogasification of corn stover through wet state NaOH pretreatment[J]. Bioresource Technology,2009,100(21):5140-5145.
[30] TAHERZADEH M J,KARIMI K.Pretreatment of lignocellulosic wastes to improve ethanol and biogas production:A review[J].International Journal of Molecular Sciences,2008,9(9):1621-1651.
[31] GAO Y S,XU J L,ZHANG Y,et al.Effects of different pretreatment methods on chemical composition of sugarcane bagasse and enzymatic hydrolysis[J].Bioresource Technology,2013,144(144C):396-400.
[32] MYAT L,RYU G H.Thermo-mechanical extrusion and sodium hydroxide pretreatments for ethanol production from destarched corn fiber[J].Environmental Progress and Sustainable Energy,2015,34(3):823-831.
[33] YOSHIDA M,LIU Y,UCHIDA S,et al.Effects of cellulose crystallinity,hemicellulose,and lignin on the enzymatic hydrolysis of Miscanthus sinensis to monosaccharides[J].Bioscience Biotechnology and Biochemistry,2008,72(3):805-810.
[34] PIHLAJANIEMI V,SIPPONEN M H,LIIMATAINEN H,et al.Weighing the factors behind enzymatic hydrolyzability of pretreated lignocellulose[J].Green Chemistry,2016,18:1295-1305.
[35] 黄富宁.碳酸钙的形貌可控制备及其稳定Pickering乳液的性能研究[D].西安:西安科技大学,2021. HUANG F N.Study on preparation of calcium carbonate with controlled morphologies and performance of stable Pickering emulsions[D].Xi’an:Xi’an University of Science and Technology,2021.(in Chinese)
[36] PRASAD R D D,REDDY M R,REDDY G V N.Effect of feeding baled and stacked urea treated rice straw on the performance of crossbred cows[J].Animal Feed Science and Technology,1998,73:347-352.

芦苇秸秆氧化钙和碳酸钠预处理工艺优化及结构分析

Process Optimization and Structure Analysis of Calcium Oxide and Sodium Carbonate Pretreatment of Reed Straw

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	刘鹏, 李静静, 乔彦良, 朱连勤, 朱风华. 黄芩多糖的提取及结构表征[J]. 中国畜牧兽医, 2023, 50(6): 2518-2530.
[2]	吴昕雨, 严琳, 伍红. 谷氨酸棒状杆菌全细胞催化合成Levan果聚糖条件优化分析[J]. 中国畜牧兽医, 2020, 47(11): 3474-3483.
[3]	丁丹, 盛小莉, 梁孔贤, 许琦, 刘卫. 伊维菌素纳米乳透皮制剂的研究[J]. , 2015, 42(2): 401-407.