钙离子载体及咖啡因对绵羊精子获能的影响

›› 2010, Vol. 37 ›› Issue (1): 112-115.

钙离子载体及咖啡因对绵羊精子获能的影响

刘烈琴，郭勇，刘云海，薛君忠，张宏，李晓燕，倪和民

（北京农学院动物科学技术系，北京 102206）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-01-20 发布日期:2010-01-20
通讯作者: 倪和民

Effects of Calcium Ionophore and Caffeine on Ovine Sperm Capacitation in vitro

LIU Lie-qin, GUO Yong, LIU Yun-hai, XUE Jun-zhong, ZHANG Hong, LI Xiao-yan, NI He-min

（Department of Animal Science & Technology, Beijing University of Agriculture, Beijng 102206, China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
Contact: NI He-min

摘要/Abstract

摘要： 通过添加不同浓度(0.05、0.1、0.2、0.5、1、5、10 μmol/L)的钙离子载体（A23187，IA）及2 mmol/L咖啡因，探讨不同作用时间其对绵羊精子体外获能的影响。结果发现，IA浓度越高，精子的体外获能率越高，顶体反应率越低，活力越低，死精子就越多；而延长作用时间对获能、顶体反应的影响不明显。IA和咖啡因共同作用后，咖啡因能够促进IA诱导顶体反应的发生。建议用IA诱导绵羊精子获能时，其浓度以0.05～0.2 μmol/L为宜，作用时间1 min。

关键词: 绵羊精子; 体外获能; 钙离子载体; 咖啡因

Abstract: It was aimed to investigate the effects of different concentrations (0.05,0.1,0.2,0.5,1,5,10 μmol/L) of calcium ionophore (A23187,IA) and 2 mmol/L caffeine on the ovine sperm capacitation in vitro.The results showed that the higher rate of capacitation in vitro， lower the rate of acrosome reaction(AR), and the lower sperm motilily were induced by the high concentration of IA, but the dead sperm could be increased. The capacitation and AR of ovine sperm in vitro were not affected even delaying time. Caffeine could promote IA to induce AR. Collectively, this might be recommol/Lended that it be applicable for improving ovine capacitation in vitro with 0.05 to 0.2 μmol/L IA for 1 min. 

Key words: ovine sperm; capacitation in vitro; calcium ionophore; caffeine

中图分类号:

S814

刘烈琴;郭勇;刘云海;薛君忠;张宏;李晓燕;倪和民. 钙离子载体及咖啡因对绵羊精子获能的影响[J]. , 2010, 37(1): 112-115.

LIU Lie-qin;GUO Yong;LIU Yun-hai;XUE Jun-zhong;ZHANG Hong;LI Xiao-yan;NI He-min. Effects of Calcium Ionophore and Caffeine on Ovine Sperm Capacitation in vitro[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(1): 112-115.

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