布鲁氏菌分子标记、毒力基因缺失株YZ-2生物学特性及免疫原性研究

›› 2010, Vol. 37 ›› Issue (2): 75-79.

布鲁氏菌分子标记、毒力基因缺失株YZ-2生物学特性及免疫原性研究

张付贤1,2，李晓艳1，杨艳玲1,2，张辉1,2，郎需龙1，唐婕1，王兴龙1

（1.军事医学科学院军事兽医研究所，长春 130062； 2.吉林大学农学部畜牧兽医学院，长春 130062）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-02-20 发布日期:2010-02-20

Research on Biological Characteristics and Immunogenicity of the Molecular Marked and Virulence Deleted Vaccine Strain YZ-2 of Brucellosis

ZHANG Fu-xian1,2, LI Xiao-yan1, YANG Yan-ling1,2, ZHANG Hui1,2, 
LANG Xu-long1, TANG Jie1, WANG Xing-long1

（1.The Military Veterinary Institute, Academy of Military Medical Sciences of PLA, Changchun 130062, China;2.College of Animal Science and Veterinary Medicine Jilin University, Changchun 130062, China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

摘要/Abstract

摘要： 摘要：试验旨在考查布鲁氏菌分子标记、毒力基因缺失疫苗株YZ-2的生物学特性和免疫原性。对YZ-2菌落形态、生化特性、变异检查试验、毒力、遗传稳定性等生物学特性，以及免疫小鼠后体液免疫和细胞免疫水平进行试验，并与其亲本株S19进行比较。结果显示，YZ-2的形态及生化特性同亲本株S19相一致，但变异检查试验结果显示YZ-2由亲本株的光滑型变为粗糙型；毒力试验证实YZ-2的毒力与亲本株S19显著减弱，且YZ-2遗传稳定性良好。免疫原性试验证实，布鲁氏菌YZ-2在整体上具有与亲本株相似的免疫原性。结果表明，布鲁氏菌分子标记、毒力基因缺失疫苗株YZ-2的安全性较S19进一步提高，稳定性好，且具有与亲本株相近的免疫原性，有很好的应用前景。

关键词: 关键词：布鲁氏菌; 分子标记疫苗; YZ2; 免疫原性

Abstract: Abstract: To determine the biological characteristics and immunogenicity of the molecular and virulence deleted vaccine strain YZ-2 of Brucellosis. The biological characteristics of Brucella abortus YZ-2 was evaluated by dyeing, toxicity, generation stability; vaccination of mice with YZ-2, the humoral immunity and cellular immunity were detected, and the results were analyzed. The research found that the biochemistry characteristics of YZ-2 and S19 were nearly identical, and the colonies and the suspension have disparity. The variation experiment proved that YZ-2 had become rough type from smooth type. Virulent experiment suggested that strain YZ-2 had lost some residual virulence of the parental strain, also YZ-2 has good heredity stability. Research verified strain YZ-2 has the immunogenicity as well as the parent strain. The results suggested that the safety of YZ-2 is better than parent strain, and the stability is well, has the same immunogenicity as parent strain; YZ-2 has good prospects to be used.

Key words: Key words: Brucella; molecular marked vaccine; YZ-2; immunogenicity

张付贤;;李晓艳;杨艳玲;;张辉;;郎需龙;唐婕;王兴龙. 布鲁氏菌分子标记、毒力基因缺失株YZ-2生物学特性及免疫原性研究[J]. , 2010, 37(2): 75-79.

ZHANG Fu-xian;;LI Xiao-yan;YANG Yan-ling;;ZHANG Hui;;LANG Xu-long;TANG Jie;WANG Xing-long. Research on Biological Characteristics and Immunogenicity of the Molecular Marked and Virulence Deleted Vaccine Strain YZ-2 of Brucellosis[J]. , 2010, 37(2): 75-79.