荷斯坦母牛AHCY基因PCR-SSCP分析

›› 2009, Vol. 36 ›› Issue (11): 93-96.

荷斯坦母牛AHCY基因PCR-SSCP分析

李拓¹，储明星²，王金玉¹，安永福³，狄冉²，方丽²

(1．扬州大学动物科学与技术学院，扬州 225009； 2．中国农业科学院北京畜牧兽医研究所，农业部畜禽遗传资源与利用重点开放实验室，北京 100193； 3．河北省畜牧兽医研究所，保定 071000)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-11-20 发布日期:2009-11-20
通讯作者: 储明星

PCR-SSCP Analysis on AHCY Gene in Holstein Cows

LI Tuo¹, CHU Ming-xing², WANG Jin-yu¹, AN Yong-fu³, DI Ran², FANG Li²

(1．College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China；2．Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China； 3．Hebei Animal Science and Veterinary Medicine Institute, Baoding 071000, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-11-20 Published:2009-11-20
Contact: CHU Ming-xing

摘要/Abstract

摘要： 根据已发表的牛AHCY基因序列设计13对引物，采用PCR-SSCP方法在210头荷斯坦母牛中检测S-腺苷高半胱氨酸水解酶（S-adenosylhomocysteine hydrolase，AHCY）基因全部10个外显子的单核苷酸多态性，同时分析该基因对荷斯坦母牛乳房炎抗性的影响。结果表明，AHCY基因的10个外显子在检测的荷斯坦母牛中都不存在多态性，AHCY基因外显子区域可能不存在影响乳房炎抗性的遗传标记。

关键词: 荷斯坦母牛; AHCY基因; PCR-SSCP

Abstract: The AHCY (S-adenosylhomocysteine hydrolase) gene was studied as a candidate gene for mastitis resistance in 210 Holstein cows. Single nucleotide polymorphisms (SNPs) of all exons (exons 1 to 10) of AHCY gene were detected with thirteen pairs of primers by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) in 210 Holstein cows. No polymorphism was detected in the amplified region in the ten exons tested, this result indicated that the genetic markers that affect the mastitis resistance in Holstein cows may not exist in the detected exons of AHCY gene.

Key words: Holstein cows; AHCY gene; PCR-SSCP

中图分类号:

李拓;储明星;王金玉;安永福;狄冉;方丽. 荷斯坦母牛AHCY基因PCR-SSCP分析 [J]. , 2009, 36(11): 93-96.

LI Tuo;CHU Ming-xing;WANG Jin-yu;AN Yong-fu;DI Ran;FANG Li. PCR-SSCP Analysis on AHCY Gene in Holstein Cows[J]. , 2009, 36(11): 93-96.

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