布鲁氏菌LPS对小鼠巨噬细胞中NLRP3炎症小体转录水平的影响

doi:10.16431/j.cnki.1671-7236.2015.01.002

›› 2015, Vol. 42 ›› Issue (1): 9-16.doi: 10.16431/j.cnki.1671-7236.2015.01.002

布鲁氏菌LPS对小鼠巨噬细胞中NLRP3炎症小体转录水平的影响

袁莎, 陈鹏博, 陈创夫

石河子大学动物科技学院, 石河子 832003

收稿日期:2014-07-25 出版日期:2015-01-20 发布日期:2015-02-06
通讯作者: 陈创夫 E-mail:ccf-xb@163.com
作者简介:袁莎(1987-), 女, 河北人, 硕士生, 研究方向:畜禽病原分子生物学
基金资助:
国家重点基础研究发展计划(973计划)项目(2010CB530203)

Effect of Brucella LPS on NLRP3-inflammasome Transcriptional Level of Mouse Macrophages

YUAN Sha, CHEN Peng-bo, CHEN Chuang-fu

College of Animal Science and Technology, Shihezi University, Shihezi 832003, China

Received:2014-07-25 Online:2015-01-20 Published:2015-02-06

摘要/Abstract

摘要： 为研究布鲁氏菌LPS对巨噬细胞中NLRP3炎症小体的影响,本试验提取布鲁氏菌2308、RB51和△WbkA的LPS,以不同浓度与小鼠巨噬细胞相互作用,荧光定量PCR检测其对NLRP3、ASC、Caspase1、IL1-β、IL18转录水平的影响.结果显示RB51 LPS和△WbkA LPS上调NLRP3炎症小体相关基因的转录水平,且呈浓度依赖性,而浓度对2308 LPS调节NLRP3炎症小体相关基因转录的作用不大;且同一浓度下,RB51 LPS和2308 LPS比△WbkA LPS更好的调节Caspase1、IL1-β、IL18的转录水平.

关键词: 布鲁氏菌LPS; 小鼠巨噬细胞; NLRP3炎症小体

Abstract: In order to find out the impact of Brucella LPS on macrophages NLRP3-inflammasome, the mouse macrophages were infected by the different concentrations of LPS which extracted from Brucella 2308, RB51 and △WbkA to explore the changes of NLRP3-inflammasome related genes, ASC, caspase1, IL1-β, IL18 at transcription levels using Real-time PCR.The results showed RB51 LPS and △WbkA LPS regulating NLRP3-inflammasome related genes transcription relied on concentrations, and concentrations of 2308 LPS had little effect on NLRP3-inflammasome related genes transcription; And under the same concentration, RB51 LPS and 2308 LPS better regulated Caspase1, IL1-β, IL18 transcriptions than △WbkA LPS.

Key words: Brucella LPS; mouse macrophage; NLRP3-inflammasome

中图分类号:

袁莎, 陈鹏博, 陈创夫. 布鲁氏菌LPS对小鼠巨噬细胞中NLRP3炎症小体转录水平的影响[J]. , 2015, 42(1): 9-16.

YUAN Sha, CHEN Peng-bo, CHEN Chuang-fu. Effect of Brucella LPS on NLRP3-inflammasome Transcriptional Level of Mouse Macrophages[J]. , 2015, 42(1): 9-16.

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布鲁氏菌LPS对小鼠巨噬细胞中NLRP3炎症小体转录水平的影响

Effect of Brucella LPS on NLRP3-inflammasome Transcriptional Level of Mouse Macrophages

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