牦牛CRH基因克隆及其在生殖轴中的表达研究

doi:10.16431/j.cnki.1671-7236.2019.08.023

Abstract

Abstract:

In order to investigate the regulation of corticotropin-releasing hormone (CRH) gene in reproduction of yak, tissue samples of hypothalamus, anterior pituitary, oviduct, ovaries and uterus were collected from 5 adult female yaks and cattle, respectively.The yak CRH gene was amplified, cloned and sequenced by RT-PCR and bioinformatics software, and phylogenetic tree was constructed.The mRNA expression in different tissues was detected by Real-time PCR.The results showed that the coding region of CRH gene in yak was 573 bp, encoding a 190 amino acid protein.The nucleotide homology of CRH gene in yak (Bos grunniens) and cattle (Bos taurus), Felis catus, Gallus gallus, Homo sapiens, Mus musculus, Rattus norvegicus and Sus scrofa was 99.8%, 43.3%, 36.1%, 46.2%, 39.0%, 38.5% and 56.4%, respectively.Phylogenetic tree results showed that yak and cattle had the closest relationship and clustered with other species, which accorded with the evolution law of species.The formula of CRH protein was C₉₂₀H₁₅₀₃N₂₇₉O₂₆₃S₃, the molecular weight and theoretical isoelectric point were 20 776.95 u and 10.95, respectively.CRH protein contained a high proportion of leucine (15.8%), proline (12.6%), alanine (12.1%) and arginine (10.5%).CRH protein was a hydrophilicity protein which contained a signal peptide and a transmembrane region.The secondary structure prediction results showed that alpha helix, beta turn, extended chain and random coil accounted for 41.05%, 1.05%, 8.42% and 49.48%, respectively, which was consistent with the tertiary structure analysis results.The expression of CRH gene mRNA in hypothalamus and uterus of yak was the highest and the lowest, respectively, and the expression of CRH gene mRNA in hypothalamus was significantly higher than that in other tissues (P<0.05).The expression of CRH gene mRNA in anterior pituitary, ovary and fallopian tube of yak were extremely significantly or significantly higher than that of cattle (P<0.01;P<0.05), but there was no significant difference in hypothalamus and uterus between two breeds (P>0.05).These results suggested that CRH gene played an important role in reproductive regulation of yak.

Key words: yak; cattle; CRH gene; cloning; tissue expression

CLC Number:

S823.8

HE Xiangdong, WANG Qin, XIA Yi, CHEN Ying, JIGE Moti, ZI Xiangdong. Cloning and Expression of Corticotropin-releasing Hormone (CRH) Gene in the Reproductive Axis of Yaks (Bos grunniens)[J]. , 2019, 46(8): 2381-2389.

References 26

[1]	WIENER G,HAN J L,LONG R J.The Yak[M].FAO Regional Office for Asia and the Pacific,2003.
[2]	ZI X D,LUO B,XIA W,et al.Characterization of transcriptional complexity during pre-implantation development of the yak ( Bos grunniens) using RNA-Seq[J]. Reproduction in Domestic Animals,2018,53(3):759-768.
[3]	殷铭阳,周东辉,刘建枝,等.中国牦牛主要寄生虫病流行现状及防控策略[J].中国畜牧兽医,2014,41(5):227-230. YIN M Y,ZHOU D H,LIU J Z,et al.Prevalence of yak main parasitic diseases in China and strategies for its control[J]. China Animal Husbandry & Veterinary Medicine,2014,41(5):227-230.(in Chinese)
[4]	石仙,熊显荣,兰道亮,等.糖对牦牛卵母细胞体外成熟及其发育能力的影响[J].中国畜牧兽医,2017,44(1):155-160. SHI X,XIONG X R,LAN D L.et al.Effect of sugar on in vitro maturation and developmental competence of yak oocytes[J]. China Animal Husbandry & Veterinary Medicine,2017,44(1):155-160.(in Chinese)
[5]	ZI X D.Reproduction in female yaks ( Bos grunniens) and opportunities for improvement[J]. Theriogenology,2003,59(5-6):1303-1312.
[6]	SUBBANNAYYA T,BALAKRISHNAN L,SUDARSHAN G,et al.An integrated map of corticotropin-releasing hormone signaling pathway[J]. Journal of Cell Communication and Signaling,2013,7(4):295-300.
[7]	GRAF C,KUEHNE C,PANHUYSEN M,et al.Corticotropin-releasing hormone regulates common target genes with divergent functions in corticotrope and neuronal cells[J]. Molecular and Cellular Endocrinology,2012,362(1-2):29-38.
[8]	YOUNG E,KORSZUN A.Psychoneuroendocrinology of depression:Hypothalamic-pituitary-gonadal axis[J]. Psychiatric Clinics of North America,1998,21(2):309-323.
[9]	VIAU V.Functional cross-talk between the hypothalamic-pituitary-gonadal and-adrenal axes[J]. Journal of Neuroendocrinology,2002,14(6):506-513.
[10]	SU F,GUO X,WANG Y,et al.Genome-wide analysis on the landscape of transcriptomes and DNA methylomes in hypothalamus reveals sexual precocity related genes in Grey goats[J]. Frontiers in Endocrinology,2018,9:501.
[11]	ROOZENDAAL M M,SWARTS J J M,WOLBERS W B,et al.Effect of CRH on the preovulatory LH and FSH surge in the cyclic rat:A role for arginine vaso-pressin?[J].Journal of Neuroendocrinology,1996,8(10):765-770.
[12]	KIAPEKOU E,ZAPANTI E,VOUKELATOU D,et al.Corticotropin-releasing hormone inhibits in vitro oocyte maturation in mice[J]. Fertility and Sterility,2011,95(4):1497-1499.
[13]	WYPIOR G,JESCHKE U,KURPISZ M,et al.Expression of CRH,CRH-related peptide and CRH receptor in the ovary and potential CRH signalling pathways[J]. Journal of Reproductive Immunology,2011,90(1):67-73.
[14]	MANOJLOVIC-STOJANOSKI M,NESTOROVIC N,TRIFUNOVIC S,et al.Dexamethasone exposure affects paraventricularnucleus and pituitary corticotrophs in female rat fetuses:An unbiased stereological and immunohistochemical study[J]. Tissue and Cell,2016,48(5):516-523.
[15]	SIMMS D,CIZDZIEL P E,CHOMCZYNSKI P.TRIzol:A new reagent for optimal single-step isolation of RNA[J]. Focus, 1993,15(4):532-535.
[16]	贾浩,张小白,宋晓峰.人类胞内蛋白半衰期与其亚细胞定位的相关性研究[J].计算机与应用化学,2011,28(4):411-414. JIA H,ZHANG X B,SONG X F.Relationship between intracellular protein half-life and subcellular localization in human cells[J]. Computers and Applied Chemistry,2011,28(4):411-414.(in Chinese)
[17]	郑海军,朱荣,葛春蕾,等.人白细胞介素-29的生物信息学分析[J].中国生物制品学杂志,2013,26(2):209-212. ZHENG H J,ZHU R,GE C L,et al.Bioinformatics of human interleukin-29[J]. Chinese Journal of Biologicals,2013,26(2):209-212.(in Chinese)
[18]	FINCH A M,YANG L G,NWAGWU M O,et al.Placental transport of leucine in a porcine model of low birth weight[J]. Reproduction,2004,128(2):229-235.
[19]	庞玉娟,杜兴雨,李强,等.玻璃化冻存对驴卵母细胞超微结构的影响[J].中国畜牧兽医,2018,45(1):147-153. PANG Y J,DU X Y,LI Q,et al.Effects of vitrification freezing on oocytes ultrastructure in Equus asinus[J]. China Animal Husbandry & Veterinary Medicine,2018,45(1):147-153.(in Chinese)
[20]	SAUTRON V,TERENINA E,GRESS L,et al.Time course of the response to ACTH in pig:Biological and transcriptomic study[J]. BMC Genomics,2015,16(1):961.
[21]	LIM L N,SUPRAMANIAM P R,MITTAL M,et al.Follicular fluid cortisol releasing hormone (CRH) levels and assisted reproductive treatment (ART) outcomes[J]. Open Journal of Obstetrics and Gynecology,2017,7(13):1271.
[22]	张蕊,常玲玲,穆春宇,等.鸽 GnIH和 GnRH基因克隆及其在不同繁殖阶段下丘脑的表达[J].中国畜牧兽医,2018,45(9):2478-2485. ZHANG R,CHANG L L,MU C Y,et al.Cloning and expression of GnIH and GnRH genes in hypothalamus of pigeons at different breeding stage[J]. China Animal Husbandry & Veterinary Medicine,2018,45(9):2478-2485.(in Chinese)
[23]	王和建,张莉,余道伦,等.BMP4诱导的Smad9在小鼠卵泡发育中的作用及其机制的初步研究[J].中国畜牧兽医,2018,45(10):2902-2909. WANG H J,ZHANG L,YU D L,et al.Preliminary study on the role of BMP4-induced Smad9 in mouse follicular development and its mechanism[J]. China Animal Husbandry & Veterinary Medicine,2018,45(10):2902-2909.(in Chinese)
[24]	CALOGERO A E,BURRELLO N,NEGRI-CESI P,et al.Effects of corticotropin-releasing hormone on ovarian estrogen production in vitro[J]. Endocrinology,1996,137(10):4161-4166.
[25]	ROGUSKI K,BARANOWSKA B,CHMIELOWSKA M,et al.Leptin modulates the corticotropin-releasing hormone (CRH) action on progesterone release from cultured rat granulosa cells[J]. Neuroendocrinology Letters,2000,21(5):383-390.
[26]	JONES J E,PICK R R,DAVENPORT M D,et al.Disinhibition of female sexual behavior by a CRH receptor antagonist in Syrian hamsters[J]. American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,2002,283(3):R591-R597.

Cloning and Expression of Corticotropin-releasing Hormone (CRH) Gene in the Reproductive Axis of Yaks (Bos grunniens)

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 26

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Rui, ZHANG Yiyang, LUO Xuehui, ZHOU Manlin, WU Shaomi, YANG Kun, QIAO Zilin. Effect of Hypoxia on Proliferation and Oxidative Stress Injury of Pulmonary Artery Smooth Muscle Cells in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2245-2254.
[2]	ZHANG Dianqi, MA Xinhao, YANG Zhimei, MEI Chugang, ZAN Linsen. Bioinformatics Analysis of PAI1 Gene and Its Effect on the Proliferation and Differentiation of Intramuscular Adipocytes in Qinchuan Beef Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1729-1741.
[3]	WU Zhijuan, CHAI Zhixin, WANG Jikun, WANG Jiabo, ZHONG Jincheng, XIN Jinwei. Expression Analysis of Specific Matrix Metalloproteinases and Functionally Related Genes of Skeletal Muscles in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1774-1784.
[4]	PENG Yangxiang, YANG Zhenbiao, YAN Kuiyou, WANG Han, HU De, WANG Zun, LIU Ning, ZHAO Liansheng. From Artificial to Intelligent:Research Progress of Individual Idendification Technology for Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1855-1866.
[5]	TAO Qinghua, HUANG Caiyun, CHEN Chao, LIU Wei, SUN Ningning, LI Ang. Cloning and Tissue Expression Difference Analysis of LIF Gene in White Muscovy Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1296-1306.
[6]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[7]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[8]	HU Leyu, HU Xinyao, LI Ying, ZHAO Shengmiao, SHEN Fengzhen, WANG Changfa, LI Liangliang, WANG Tongtong, REN Huiying. Bioinformatics Analysis of HO-1 Gene and Preparation of Polyclonal Antibody in Dezhou Donkey [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1499-1510.
[9]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[10]	WANG Yunlu, MENG Zhaoyi, YAO Yilong, GUO Min, NIU Jiaqaing, SUOLANG Sizhu, XU Yefen. Screening and Targeting Verification of lncRNA of "Sponge Adsorption" bta-miR-146a in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 859-869.
[11]	LI Dan, ZHANG Haisen, WANG Yiqun, GAO Dengke, ZHAO Hongcong, LI Chao, JIN Yaping, CHEN Huatao. Eukaryotic Expression Vector Construction,Bioinformatics and Tissue Expression Profiles Analysis of CRY1 Gene in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 870-881.
[12]	ZHANG Yongjie, SUI Zhiyuan, ZHANG Zhishuai, WANG Chenguang, LI Xiaojun, XING Feng. Cloning and Bioinformatics Analysis of NPTX1 Gene and Its Tissues Expression at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1048-1058.
[13]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[14]	LI Mingbo, GAO Qingshan, LI Guanhao, ZHANG Kui, CUI Lianhua, HOU Lina. Effects of Chinese Herbal Medicine Feed Additives on Meat Quality and Flavor Substances in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 531-542.
[15]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.