驴Zfx基因CDS序列克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2019.06.017

Abstract

Abstract:

This study was aimed to clone the CDS sequence of Zfx gene in Equus asinus and analyze the sequence by bioinformatics softwares.Specific primers were designed according to the mRNA sequence of Zfx gene in Bos taurus published in GenBank (accession No.:D84097.1).The CDS sequence of Zfx gene in Equus asinus was gained by RT-PCR,and the nucleotide sequence and protein structure prediction of Zfx gene CDS in Equus asinus were analyzed.The results showed that the CDS sequence of Zfx gene in Equus asinus was 2 403 bp,encoding 800 amino acids.The nucleotide sequence homology of Zfx gene CDS sequence in Equus asinus with Equus caballus,Bos taurus,Canis lupus familiaris,Ceratotherium simum,Cervus elaphus,Feils catus,Homo sapiens,Ovis aries and Pan troglodytes were 99.6%,95.0%,95.3%,97.9%,93.5%,94.9%,95.0%,95.7% and 94.9%,respectively.Phylogenetic tree was constructed for nucleotide sequences of Zfx gene CDS in Equus asinuss and other 9 mammals,which showed that Equus asinus were closely related to Equus caballus,followed by genetic relationship with Ceratotherium simum,and they were closely related to Cants lupus familiaris and Feils catus that were in the same class,and the genetic relationship was week with Ovis aries and Bos taurus.The theoretical isoelectric point (pI) of Zfx protein was 5.19,the instability coefficient was 42.94,and the extinction coefficient was 35 810.Zfx protein belonged to unstable hydrophilic protein and non-secreted protein,contained 60 phosphorylation sites,no signal peptide and transmembrane structure.The alpha helix,extended chain and random coil of Zfx protein were 23.12%,20.25% and 56.63%,respectively,and the tertiary structure was mainly alpha helix and random coil.In this experiment,the CDS sequence of Zfx gene in Equus asinus was successfully cloned,which provided a basis for further study on the structural function of Zfx gene and its enconded protein.

Key words: Equus asinus; Zfxgene; cloning; sequence analysis

CLC Number:

Q785

ZHANG Zhidong, XIA Huan, ZOU Shenglei, AN Hongyong, LI Chaocheng, JIA Bin. Cloning and Sequence Analysis of Zfx Gene CDS Sequence in Equus asinus[J]. , 2019, 46(6): 1704-1712.

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Cloning and Sequence Analysis of Zfx Gene CDS Sequence in Equus asinus

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