L-NMMA对鸡球虫早熟弱毒疫苗免疫鸡增重的影响

doi:10.16431/j.cnki.1671-7236.2019.03.027

Abstract

Abstract:

In order to study the effect of chicken coccidia premature attenuated vaccine and nitric oxide synthase inhibitor (L-NMMA) on the weight gain of immunized chicken,and the effect of L-NMMA on immune efficacy.Forty SPF chicks were divided into two blank control groups (C1 and C2) and two single-dose repeated inoculated groups(T1 and T2).In groups T1 and T2,chicks were inoculated repeatedly with the premature attenuated vaccine of chicken coccidia at the recommended single dose,then the chicks with empty stomach were weighted separately on 7th and 14th after the second inoculated.The duodenum,jejunum and cecum lesions were recorded.The heart,liver,spleen,lung,kidney,glandular stomach,muscular stomach,duodenum,jejunum and cecum of the chicks were collected for histopathological examination.Another forty SPF chicks were divided into blank control group (C20),challenge control group (T21),immune control group (T22) and immune with drug group (T23).In groups T22 and T23,chicks were vaccinated twice with premature attenuated vaccine at the recommended immune dose,and the chicks in T23 group were injected with L-NMMA at the same time.On the 14th after the second immune,the chicks were gavaged with mixed virulent chicken coccidia,then the average weight gain,relative weight gain,intestonal (duodenun,jejunum and cecum) lesion scores,reduction of lesion scores (RLS),bloody stool scores,mortality and oocyst production were measured.The results showed that the average weight gain of the vaccinated group was significantly lower than the unvaccinated group (P<0.05).The duodenum,jejunum and cecum of the vaccinated group had mild lesions and the mucosal epithelial cells had different degrees of damage and shedding.The average weight gains of the vaccinated with L-NMMA group after the first,second immuned and challenged were all extremely significantly higher than the immune-challenged control group (P<0.01),while the average weight gain was not significantly different with the blank control group (P>0.05),and there were no significant differences in intestinal lesion score and oocyst yield between immune control group and drug immune group (P>0.05).The results demonstrated that premature attenuated vaccine of chicken coccidia had light damage to the intestines of the chicks and had certain negative effects on the weight gain.In addition,L-NMMA could reduce the vaccine’s adverse effect on the weight gain and had no effect on the immune efficacy.

Key words: chicken coccidia premature attenuated vaccine; chicken coccidiosis; L-NMMA; weight gain

CLC Number:

S852.72⁺3

FENG Yuping, HAN Tianfei, LYU Qianghua, MI Chenglong, HAO Feifei, ZHANG Li, ZHANG Xuesong, ZHENG Mingxue. Effect of L-NMMA on Weight Gain of Chickens Infected with Chicken Coccidia Premature Attenuated Vaccine[J]. , 2019, 46(3): 865-872.

References

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Effect of L-NMMA on Weight Gain of Chickens Infected with Chicken Coccidia Premature Attenuated Vaccine

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