牦牛FSHR基因克隆及其在生殖轴中的表达研究

doi:10.16431/j.cnki.1671-7236.2019.02.001

Abstract

Abstract:

The aim of this study was to investigate the potential role of follicle stimulating hormone receptor (FSHR) in regulating yak reproduction by clarifying its CDS sequence and expression characteristics in the reproductive axis of yak (Bos grunniens).The hypothalamus,anterior pituitary,ovary,oviduct and uterus of yak and cattle (Bos taurus) were collected during the follicular phase.The yak FSHR gene was amplified and cloned by RT-PCR,and its mRNA expression levels in different tissues was detected by Real-time PCR.The results showed that the FSHR coding region of the yak was 2 088 bp,encoding 695 amino acids.The formula of FSHR protein was C₆₃₇₈H₁₀₆₇₀N₂₀₈₈O₂₆₃₇S₅₇₆,the molecular weight of FSHR protein was 177 263.85 u,and the theoretical isoelectric point was 4.88.Yak FSHR amino acids shared high homology with cattle,sheep,goat and wild boars (91.50% to 99.38%).The FSHR protein was an acid labile hydrophobin protein with 8 transmembrane structures and signal peptides;The secondary structure consisted of extended chain (15.54%),α-helix (42.30%),β-turn (1.44%) and random coil (40.72%).The phylogenetic tree showed the genetic relationship was the closest between yak and cattle.The Real-time PCR results showed that FSHR gene expressed in all tissues examined.Its expression in yak uterus was significantly or extremely significantly higher than other tissues (except ovary)(P<0.05;P<0.01),while it was significantly of extremely significantly higher in ovary of cattle than other tissues (P<0.05;P<0.01).Its expression level in cattle ovary was extremely significantly higher than yak (P<0.01).These data indicated that FSHR gene was relatively conservative in the course of animal evolution,and its lower expression in yak ovary possibly affected yak reproduction.

Key words: yak; cattle; FSHR gene; cloning; tissue expression

CLC Number:

S823.8⁺5

XIA Yi, WANG Qin, HE Xiangdong, CHEN Ying, JIGE Moti, ZI Xiangdong. Cloning and Expression Analysis in Reproductive Axis of Yak FSHR Gene[J]. , 2019, 46(2): 329-337.

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Cloning and Expression Analysis in Reproductive Axis of Yak FSHR Gene

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